Modeling Hematologic and Biochemical Parameters with Spatiotemporal Analysis for the Free-Ranging Eastern Box Turtle (Terrapene carolina carolina) in Illinois and Tennessee, a Potential Biosentinel

Terrell C. Lloyd, Matthew C Allender, Grace Archer, Christopher A Phillips, John Byrd, A. Russell Moore

Research output: Contribution to journalArticle

Abstract

Box turtles are long-lived, inhabit both aquatic and terrestrial habitats, and have relatively small home ranges making them a suitable candidate as a sentinel. To characterize their changes in response to environmental health, assessment of observed variation of this species is required. Thus, a comparative health assessment was employed in 825 Eastern box turtles in east central Illinois and Oak Ridge, Tennessee, to establish a baseline health assessment with regard to sex, age class, season, and location, identify temporal trends, and map parameters. Hematological and plasma biochemical variables measured included packed cell volume, total solids, white blood cell and differential counts, calcium, phosphorus, aspartate aminotransferase, bile acids, creatine kinase, and uric acid. These variables were reduced to four principle components that explained 68.8 % of the cumulative variance. The top model included the main effects of year, location, and sex, but no interactions. Spatial analysis of turtles in Tennessee demonstrated increased WBC and decreased PCV in 2011 associated with a clear-cut silviculture site. The results of this health assessment can serve as a baseline of population health in future studies and aid in the utility of this species as a sentinel.

Original languageEnglish (US)
Pages (from-to)467-479
Number of pages13
JournalEcoHealth
Volume13
Issue number3
DOIs
StatePublished - Sep 1 2016

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spatiotemporal analysis
Spatio-Temporal Analysis
Turtles
turtle
Health
modeling
Homing Behavior
Spatial Analysis
silviculture
Environmental Health
acid
Creatine Kinase
Aspartate Aminotransferases
Uric Acid
Bile Acids and Salts
home range
age class
Leukocyte Count
Cell Size
spatial analysis

Keywords

  • biosentinel
  • chelonian
  • environment
  • health
  • reptile

ASJC Scopus subject areas

  • Ecology
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Modeling Hematologic and Biochemical Parameters with Spatiotemporal Analysis for the Free-Ranging Eastern Box Turtle (Terrapene carolina carolina) in Illinois and Tennessee, a Potential Biosentinel",
abstract = "Box turtles are long-lived, inhabit both aquatic and terrestrial habitats, and have relatively small home ranges making them a suitable candidate as a sentinel. To characterize their changes in response to environmental health, assessment of observed variation of this species is required. Thus, a comparative health assessment was employed in 825 Eastern box turtles in east central Illinois and Oak Ridge, Tennessee, to establish a baseline health assessment with regard to sex, age class, season, and location, identify temporal trends, and map parameters. Hematological and plasma biochemical variables measured included packed cell volume, total solids, white blood cell and differential counts, calcium, phosphorus, aspartate aminotransferase, bile acids, creatine kinase, and uric acid. These variables were reduced to four principle components that explained 68.8 {\%} of the cumulative variance. The top model included the main effects of year, location, and sex, but no interactions. Spatial analysis of turtles in Tennessee demonstrated increased WBC and decreased PCV in 2011 associated with a clear-cut silviculture site. The results of this health assessment can serve as a baseline of population health in future studies and aid in the utility of this species as a sentinel.",
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