Miscanthus × giganteus xylooligosaccharides: Purification and fermentation

Ming Hsu Chen, Michael J. Bowman, Michael A. Cotta, Bruce S. Dien, Loren B. Iten, Terence R. Whitehead, Kent D. Rausch, M. E. Tumbleson, Vijay Singh

Research output: Contribution to journalArticlepeer-review


A procedure was developed to recover xylooligosaccharides (XOS) from Miscanthus × giganteus (M × G) hydrolyzate. M × G hydrolyzate was prepared using autohydrolysis, and XOS rich fractions were acquired using activated carbon adsorption and stepwise ethanol elution. The combined XOS fractions were purified using a series of ion exchange resin treatments. The end product, M × G XOS, had 89.1% (w/w) total substituted oligosaccharides (TSOS) composed of arabinose, glucose, xylose and acetyl group. Bifidobacterium adolescentis and Bifidobacterium catenulatum (health promoting bacteria) were cultured in vitro on M × G XOS and a commercial XOS source, which was used as a comparison. B. adolescentis grew to a higher cell density than B. catenulatum in both XOS cultures. Total xylose consumption for B. adolescentis was 84.1 and 84.8%, respectively for M × G and commercial XOS cultures; and for B. catenulatum was 76.6 and 73.6%, respectively. The xylobiose (X2), xylotriose (X3) and xylotetraose (X4) were almost utilized for both strains. Acetic and lactic acids were the major fermentation products of the XOS cultures.

Original languageEnglish (US)
Pages (from-to)96-103
Number of pages8
JournalCarbohydrate Polymers
StatePublished - Apr 20 2016


  • Acetic acid
  • Autohydrolysis
  • Fermentation
  • Lactic acid
  • Miscanthus × giganteus
  • Xylooligosaccharides

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry


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