MIR100 host gene-encoded lncRNAs regulate cell cycle by modulating the interaction between HuR and its target mRNAs

Qinyu Sun, Vidisha Tripathi, Je Hyun Yoon, Deepak K. Singh, Qinyu Hao, Kyung Won Min, Sylvia Davila, Richard W. Zealy, Xiao Ling Li, Maria Polycarpou-Schwarz, Elin Lehrmann, Yongqing Zhang, Kevin G. Becker, Susan M. Freier, Yuelin Zhu, Sven Diederichs, Supriya Gangadharan Prasanth, Ashish Lal, Myriam Gorospe, Prasanth Kumar Kannanganattu

Research output: Contribution to journalArticle

Abstract

Long non-coding RNAs (lncRNAs) regulate vital biological processes, including cell proliferation, differentiation and development. A subclass of lncRNAs is synthesized from microRNA (miRNA) host genes (MIRHGs) due to pre-miRNA processing, and are categorized as miRNA-host gene lncRNAs (lnc-miRHGs). Presently, the cellular function of most lnc-miRHGs is not well understood. We demonstrate a miRNA-independent role for a nuclear-enriched lnc-miRHG in cell cycle progression. MIR100HG produces spliced and stable lncRNAs that display elevated levels during the G1 phase of the cell cycle. Depletion of MIR100HG-encoded lncRNAs in human cells results in aberrant cell cycle progression without altering the levels of miRNA encoded within MIR100HG. Notably, MIR100HG interacts with HuR/ELAVL1 as well as with several HuR-target mRNAs. Further, MIR100HG-depleted cells show reduced interaction between HuR and three of its target mRNAs, indicating that MIR100HG facilitates interaction between HuR and target mRNAs. Our studies have unearthed novel roles played by a MIRHG-encoded lncRNA in regulating RNA binding protein activity, thereby underscoring the importance of determining the function of several hundreds of lnc-miRHGs that are present in human genome.

Original languageEnglish (US)
Pages (from-to)10405-10416
Number of pages12
JournalNucleic acids research
Volume46
Issue number19
DOIs
StatePublished - Jan 1 2018

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Long Noncoding RNA
MicroRNAs
Cell Cycle
Messenger RNA
Genes
Biological Phenomena
RNA-Binding Proteins
G1 Phase
Human Genome
Cell Differentiation
Cell Proliferation

ASJC Scopus subject areas

  • Genetics

Cite this

MIR100 host gene-encoded lncRNAs regulate cell cycle by modulating the interaction between HuR and its target mRNAs. / Sun, Qinyu; Tripathi, Vidisha; Yoon, Je Hyun; Singh, Deepak K.; Hao, Qinyu; Min, Kyung Won; Davila, Sylvia; Zealy, Richard W.; Li, Xiao Ling; Polycarpou-Schwarz, Maria; Lehrmann, Elin; Zhang, Yongqing; Becker, Kevin G.; Freier, Susan M.; Zhu, Yuelin; Diederichs, Sven; Prasanth, Supriya Gangadharan; Lal, Ashish; Gorospe, Myriam; Kannanganattu, Prasanth Kumar.

In: Nucleic acids research, Vol. 46, No. 19, 01.01.2018, p. 10405-10416.

Research output: Contribution to journalArticle

Sun, Q, Tripathi, V, Yoon, JH, Singh, DK, Hao, Q, Min, KW, Davila, S, Zealy, RW, Li, XL, Polycarpou-Schwarz, M, Lehrmann, E, Zhang, Y, Becker, KG, Freier, SM, Zhu, Y, Diederichs, S, Prasanth, SG, Lal, A, Gorospe, M & Kannanganattu, PK 2018, 'MIR100 host gene-encoded lncRNAs regulate cell cycle by modulating the interaction between HuR and its target mRNAs', Nucleic acids research, vol. 46, no. 19, pp. 10405-10416. https://doi.org/10.1093/nar/gky696
Sun, Qinyu ; Tripathi, Vidisha ; Yoon, Je Hyun ; Singh, Deepak K. ; Hao, Qinyu ; Min, Kyung Won ; Davila, Sylvia ; Zealy, Richard W. ; Li, Xiao Ling ; Polycarpou-Schwarz, Maria ; Lehrmann, Elin ; Zhang, Yongqing ; Becker, Kevin G. ; Freier, Susan M. ; Zhu, Yuelin ; Diederichs, Sven ; Prasanth, Supriya Gangadharan ; Lal, Ashish ; Gorospe, Myriam ; Kannanganattu, Prasanth Kumar. / MIR100 host gene-encoded lncRNAs regulate cell cycle by modulating the interaction between HuR and its target mRNAs. In: Nucleic acids research. 2018 ; Vol. 46, No. 19. pp. 10405-10416.
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abstract = "Long non-coding RNAs (lncRNAs) regulate vital biological processes, including cell proliferation, differentiation and development. A subclass of lncRNAs is synthesized from microRNA (miRNA) host genes (MIRHGs) due to pre-miRNA processing, and are categorized as miRNA-host gene lncRNAs (lnc-miRHGs). Presently, the cellular function of most lnc-miRHGs is not well understood. We demonstrate a miRNA-independent role for a nuclear-enriched lnc-miRHG in cell cycle progression. MIR100HG produces spliced and stable lncRNAs that display elevated levels during the G1 phase of the cell cycle. Depletion of MIR100HG-encoded lncRNAs in human cells results in aberrant cell cycle progression without altering the levels of miRNA encoded within MIR100HG. Notably, MIR100HG interacts with HuR/ELAVL1 as well as with several HuR-target mRNAs. Further, MIR100HG-depleted cells show reduced interaction between HuR and three of its target mRNAs, indicating that MIR100HG facilitates interaction between HuR and target mRNAs. Our studies have unearthed novel roles played by a MIRHG-encoded lncRNA in regulating RNA binding protein activity, thereby underscoring the importance of determining the function of several hundreds of lnc-miRHGs that are present in human genome.",
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AU - Sun, Qinyu

AU - Tripathi, Vidisha

AU - Yoon, Je Hyun

AU - Singh, Deepak K.

AU - Hao, Qinyu

AU - Min, Kyung Won

AU - Davila, Sylvia

AU - Zealy, Richard W.

AU - Li, Xiao Ling

AU - Polycarpou-Schwarz, Maria

AU - Lehrmann, Elin

AU - Zhang, Yongqing

AU - Becker, Kevin G.

AU - Freier, Susan M.

AU - Zhu, Yuelin

AU - Diederichs, Sven

AU - Prasanth, Supriya Gangadharan

AU - Lal, Ashish

AU - Gorospe, Myriam

AU - Kannanganattu, Prasanth Kumar

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Long non-coding RNAs (lncRNAs) regulate vital biological processes, including cell proliferation, differentiation and development. A subclass of lncRNAs is synthesized from microRNA (miRNA) host genes (MIRHGs) due to pre-miRNA processing, and are categorized as miRNA-host gene lncRNAs (lnc-miRHGs). Presently, the cellular function of most lnc-miRHGs is not well understood. We demonstrate a miRNA-independent role for a nuclear-enriched lnc-miRHG in cell cycle progression. MIR100HG produces spliced and stable lncRNAs that display elevated levels during the G1 phase of the cell cycle. Depletion of MIR100HG-encoded lncRNAs in human cells results in aberrant cell cycle progression without altering the levels of miRNA encoded within MIR100HG. Notably, MIR100HG interacts with HuR/ELAVL1 as well as with several HuR-target mRNAs. Further, MIR100HG-depleted cells show reduced interaction between HuR and three of its target mRNAs, indicating that MIR100HG facilitates interaction between HuR and target mRNAs. Our studies have unearthed novel roles played by a MIRHG-encoded lncRNA in regulating RNA binding protein activity, thereby underscoring the importance of determining the function of several hundreds of lnc-miRHGs that are present in human genome.

AB - Long non-coding RNAs (lncRNAs) regulate vital biological processes, including cell proliferation, differentiation and development. A subclass of lncRNAs is synthesized from microRNA (miRNA) host genes (MIRHGs) due to pre-miRNA processing, and are categorized as miRNA-host gene lncRNAs (lnc-miRHGs). Presently, the cellular function of most lnc-miRHGs is not well understood. We demonstrate a miRNA-independent role for a nuclear-enriched lnc-miRHG in cell cycle progression. MIR100HG produces spliced and stable lncRNAs that display elevated levels during the G1 phase of the cell cycle. Depletion of MIR100HG-encoded lncRNAs in human cells results in aberrant cell cycle progression without altering the levels of miRNA encoded within MIR100HG. Notably, MIR100HG interacts with HuR/ELAVL1 as well as with several HuR-target mRNAs. Further, MIR100HG-depleted cells show reduced interaction between HuR and three of its target mRNAs, indicating that MIR100HG facilitates interaction between HuR and target mRNAs. Our studies have unearthed novel roles played by a MIRHG-encoded lncRNA in regulating RNA binding protein activity, thereby underscoring the importance of determining the function of several hundreds of lnc-miRHGs that are present in human genome.

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