Miniaturized Plate Readers for Low-Cost, High-Throughput Phenotypic Screening

Paul A. Jensen, Bonnie V. Dougherty, Thomas J. Moutinho, Jason A. Papin

Research output: Contribution to journalArticlepeer-review

Abstract

We present a miniaturized plate reader for measuring optical density in 96-well plates. Our standalone reader fits in most incubators, environmental chambers, or biological containment suites, allowing users to leverage their existing laboratory infrastructure. The device contains no moving parts, allowing an entire 96-well plate to be read several times per second. We demonstrate how the fast sampling rate allows our reader to detect small changes in optical density, even when the device is placed in a shaking incubator. A wireless communication module allows remote monitoring of multiple devices in real time. These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalJournal of Laboratory Automation
Volume20
Issue number1
DOIs
StatePublished - Feb 24 2015
Externally publishedYes

Keywords

  • growth curve
  • multiwell plate assays
  • phenotypic screening
  • plate reader

ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology

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