Milk Fat Globule Membrane Protects Lactobacillus rhamnosus GG from Bile Stress by Regulating Exopolysaccharide Production and Biofilm Formation

Lili Zhang, Lili Zhang, Lili Zhang, Maciej Chichlowski, Maciej Chichlowski, Gabriele Gross, Gabriele Gross, Maxwell J. Holle, Luis A. Lbarra-Sánchez, Shumei Wang, Michael J. Miller

Research output: Contribution to journalArticlepeer-review

Abstract

The milk fat globule membrane (MFGM) is a complex, highly conserved structure surrounding fat droplets secreted into mammalian milk. This study evaluated the impact of MFGM on Lactobacillus rhamnosus GG (LGG). MFGM-10 (2.5 g/L, 5 g/L, and 10 g/L) did not affect LGG growth in MRS medium but enhanced the ability of LGG to survive in the presence of 0.5% porcine bile. In the presence of MFGM-10 (5 g/L) and bile (0.5%), there were less complex polysaccharides in the media and less capsular polysaccharides associated with the LGG cells compared to the bile exposure alone (p < 0.05). The expression of four EPS genes was modulated by bile stress and MFGM. Biofilm thickness was increased (p < 0.05) during bile stress with MFGM compared to other treatments. Furthermore, MFGM increased LGG survival during transit in the murine GI tract. Future experiments will determine the impact of MFGM on LGG probiotic functionality.

Original languageEnglish (US)
Pages (from-to)6646-6655
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume68
Issue number24
DOIs
StatePublished - Jun 17 2020

Keywords

  • Lactobacillus rhamnosus GG
  • MFGM
  • bile resistance
  • biofilm formation
  • exopolysaccharide
  • milk fat globule membrane
  • probiotics

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Milk Fat Globule Membrane Protects Lactobacillus rhamnosus GG from Bile Stress by Regulating Exopolysaccharide Production and Biofilm Formation'. Together they form a unique fingerprint.

Cite this