Microstamp patterns of biomolecules for high-resolution neuronal networks

D. W. Branch, J. M. Corey, J. A. Weyhenmeyer, G. J. Brewer, B. C. Wheeler

Research output: Contribution to journalArticlepeer-review


A microstamping technique has been developed for high-resolution patterning of proteins on glass substrates for the localisation of neurons and their axons and dendrites. The patterning process uses a microfabricated polydimethylsiloxane stamp with micrometer length features to transfer multiple types of biomolecules to silane-derivatised substrates, using glutaraldehyde as a homobifunctional linker. To test the efficacy of the procedure, substrates are compared in which poly-d-lysine (PDL) was physisorbed and patterned by photoresist with those stamped with PDL. Fluorescein isothiocyanate labelled poly-l-lysine was used to verify the presence and uniformity of the patterns on the glass substrates. As a biological assay, B104 neuroblastoma cells were plated on stamped and physisorbed glass coverslips. Pattern compliance was determined as the percentage of cells on the pattern 8 h after plating. Results indicate that the stamping and photoresist patterning procedure are equivalent. Substrates stamped with PDL had an average pattern compliance of 52.6 ± 4.4%, compared to 54.6 ± 8.1% for physisorbed substrates. Measures of background avoidance were also equivalent. As the procedure permits successive stamping of multiple proteins, each with its own micro-pattern, it should be very useful for defining complex substrates to assist in cell patterning and other cell guidance studies.

Original languageEnglish (US)
Pages (from-to)135-141
Number of pages7
JournalMedical and Biological Engineering and Computing
Issue number1
StatePublished - Jan 1998


  • B104
  • Micro
  • Microstamp
  • Neural culture
  • Pattern
  • Stamp

ASJC Scopus subject areas

  • Biomedical Engineering
  • Computer Science Applications


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