Microscopic mechanics of hairpin DNA translocation through synthetic nanopores

Jeffrey Comer, Valentin Dimitrov, Qian Zhao, Gregory Timp, Aleksei Aksimentiev

Research output: Contribution to journalArticle

Abstract

Nanoscale pores have proved useful as a means to assay DNA and are actively being developed as the basis of genome sequencing methods. Hairpin DNA (hpDNA), having both double-helical and overhanging coil portions, can be trapped in a nanopore, giving ample time to execute a sequence measurement. In this article, we provide a detailed account of hpDNA interaction with a synthetic nanopore obtained through extensive all-atom molecular dynamics simulations. For synthetic pores with minimum diameters from 1.3 to 2.2 nm, we find that hpDNA can translocate by three modes: unzipping of the double helix and - in two distinct orientations - stretching/distortion of the double helix. Furthermore, each of these modes can be selected by an appropriate choice of the pore size and voltage applied transverse to the membrane. We demonstrate that the presence of hpDNA can dramatically alter the distribution of ions within the pore, substantially affecting the ionic current through it. In experiments and simulations, the ionic current relative to that in the absence of DNA can drop below 10% and rise beyond 200%. Simulations associate the former with the double helix occupying the constriction and the latter with accumulation of DNA that has passed through the constriction.

Original languageEnglish (US)
Pages (from-to)593-608
Number of pages16
JournalBiophysical journal
Volume96
Issue number2
DOIs
StatePublished - Jan 21 2009

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'Microscopic mechanics of hairpin DNA translocation through synthetic nanopores'. Together they form a unique fingerprint.

  • Cite this