Abstract

Optical contrast based on elastic scattering interactions between light and matter can be used to probe cellular structure, cellular dynamics, and image tissue architecture. The quantitative nature and high sensitivity of light scattering signals to subtle alterations in tissue morphology, as well as the ability to visualize unstained tissue in vivo, has recently generated significant interest in optical-scatter-based biosensing and imaging. Here we review the fundamental methodologies used to acquire and interpret optical scatter data. We report on recent findings in this field and present current advances in optical scatter techniques and computational methods. Cellular and tissue data enabled by current advances in optical scatter spectroscopy and imaging stand to impact a variety of biomedical applications including clinical tissue diagnosis, in vivo imaging, drug discovery, and basic cell biology.

Original languageEnglish (US)
Pages (from-to)285-314
Number of pages30
JournalAnnual Review of Biomedical Engineering
Volume12
DOIs
StatePublished - Aug 15 2010

Fingerprint

Spectrum Analysis
Spectroscopy
Tissue
Imaging techniques
Light
Cytology
Photophobia
Elastic scattering
Cellular Structures
Drug Discovery
Computational methods
Light scattering
Cell Biology

Keywords

  • cell analysis
  • light scattering
  • microscopy
  • optical imaging
  • tissue diagnosis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Microscopic imaging and spectroscopy with scattered light. / Boustany, Nada N.; Boppart, Stephen A.; Backman, Vadim.

In: Annual Review of Biomedical Engineering, Vol. 12, 15.08.2010, p. 285-314.

Research output: Contribution to journalReview article

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