MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting the INSIG1 gene

Hui Wang, Jun Luo, Tianying Zhang, Huibin Tian, Yue Ma, Huifen Xu, Dawei Yao, Juan J. Loor

Research output: Contribution to journalArticle

Abstract

The microRNA-26 (miR-26) family is known to control adipogenesis in non-ruminants. The genomic loci of miR-26a and miR-26b have been localized in the introns of genes encoding for the proteins of the Cterminal domain RNA polymerase II polypeptide A small phosphatase (CTDSP) family. Insulin-induced gene 1 (INSIG1) encodes a protein with a key role in the regulation of lipogenesis in rodent liver. In the present study, we investigated the synergistic function of the miR-26 family and their host genes in goat mammary epithelial cells (GMEC). Downregulation of miR-26a/b and their host genes in GMEC decreased the expression of genes relate to fatty acid synthesis (PPARG, LXRA, SREBF1, FASN, ACACA, GPAM, LPIN1, DGAT1 and SCD1), triacylglycerol accumulation and unsaturated fatty acid synthesis. Luciferase reporter assays confirmed INSIG1 as a direct target of miR-26a/b. Furthermore, inhibition of the CTDSP family also downregulated the expression of INSIG1. Taken together, our findings highlight a functional association of miR-26a/b, their host genes and INSIG1, and provide new insights into the regulatory network controlling milk fat synthesis in GMEC. The data indicate that targeting this network via nutrition might be important for regulating milk fat synthesis in ruminants.

Original languageEnglish (US)
Pages (from-to)500-510
Number of pages11
JournalRNA Biology
Volume13
Issue number5
DOIs
StatePublished - Apr 13 2016

Keywords

  • CTDSP family
  • INSIG1
  • MicroRNA-26
  • TAG accumulation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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