Microproteomics: Analysis of protein diversity in small samples

Howard B. Gutstein, Jeffrey S. Morris, Suresh P. Annangudi, Jonathan V. Sweedler

Research output: Contribution to journalArticlepeer-review


Proteomics, the large-scale study of protein expression in organisms, offers the potential to evaluate global changes in protein expression and their post-translational modifications that take place in response to normal or pathological stimuli. One challenge has been the requirement for substantial amounts of tissue in order to perform comprehensive proteomic characterization. In heterogeneous tissues, such as brain, this has limited the application of proteomic methodologies. Efforts to adapt standard methods of tissue sampling, protein extraction, arraying, and identification are reviewed, with an emphasis on those appropriate to smaller samples ranging in size from several microliters down to single cells. The effects of miniaturization on these analyses are highlighted using neuroscience-related examples, as are statistical issues unique to the high-dimensional datasets generated by proteomic experiments.

Original languageEnglish (US)
Pages (from-to)316-330
Number of pages15
JournalMass Spectrometry Reviews
Issue number4
StatePublished - Jul 2008


  • 2d gel electmphoresis
  • Capillary electrophoresis
  • Imaging mass spectrometry
  • Microdissection

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Analytical Chemistry
  • Spectroscopy
  • Biochemistry, Genetics and Molecular Biology(all)


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