Abstract
This review describes a new type of label-free optical biosensor that is inexpensively manufactured from continuous sheets of plastic film and incorporated into standard format microplates to enable highly sensitive, high-throughput detection of small molecules, proteins and cells. The biosensor and associated detection instrumentation are applied to review two fundamental limiting issues for assays in proteomics research and drug discovery: requirement for quantitative measurement of protein concentration and specific activity, and measurements made with complex systems in highly parallel measurements. SRU BIosystems, Inc.'s BIND™ label-free detection will address these issues using data examples for hybridoma screening, epitope binning and mapping, small-molecule screening, and cell-based functional assays. The review describes several additional applications that are under development for the system, and the key issues that will drive adoption of the technology over the next 5 years.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 271-281 |
| Number of pages | 11 |
| Journal | Expert Review of Proteomics |
| Volume | 3 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2006 |
Keywords
- Biomolecular interaction
- High-throughput screening
- Label-free detection
- Optical biosensor
- Photonic crystal
- Proteomics
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Biotechnology