Microplate-based, label-free detection of biomolecular interactions: Applications in proteomics

Brian T. Cunningham, Lance Laing

Research output: Contribution to journalReview article

Abstract

This review describes a new type of label-free optical biosensor that is inexpensively manufactured from continuous sheets of plastic film and incorporated into standard format microplates to enable highly sensitive, high-throughput detection of small molecules, proteins and cells. The biosensor and associated detection instrumentation are applied to review two fundamental limiting issues for assays in proteomics research and drug discovery: requirement for quantitative measurement of protein concentration and specific activity, and measurements made with complex systems in highly parallel measurements. SRU BIosystems, Inc.'s BIND™ label-free detection will address these issues using data examples for hybridoma screening, epitope binning and mapping, small-molecule screening, and cell-based functional assays. The review describes several additional applications that are under development for the system, and the key issues that will drive adoption of the technology over the next 5 years.

Original languageEnglish (US)
Pages (from-to)271-281
Number of pages11
JournalExpert Review of Proteomics
Volume3
Issue number3
DOIs
StatePublished - Jul 7 2006

Keywords

  • Biomolecular interaction
  • High-throughput screening
  • Label-free detection
  • Optical biosensor
  • Photonic crystal
  • Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Biotechnology

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