Micropatterning B104 neuroblastoma cells in culture: An assay for neuronal pattern synthesis

Bruce C. Wheeler, Joseph M. Corey, Anna L. Brunette, Michael S. Chen, James A. Weyhenmeyer

Research output: Contribution to journalConference articlepeer-review

Abstract

Our goal is the reliable synthesis of patterned living neural networks. To assess rapidly the qualities of candidate substrates we use the B104 neuroblastoma cell line with and without the differentiating agent agent dibutyrylcyclicAMP (DBcAMP). B104 cells prefer, in order, poly-D-lysine (PDL), to phenyltrichlorosilane (PTCS), coverslip glass, and silicon dioxide coated coverslips. At 8 hours B104 cells on micropatterned had excellent compliance of somata (86%), but not of neurites (10% of background areas free of neurites). Compliance was greatly reduced thereafter. Addition of DBcAMP increased the compliance of neurites (70%), with patterns maintained up to 72 hours. The assay identified chondroitin sulfate (CSPG) as more cytophobic than mouse fibrinogen, bovine serum albumin (BSA), and glycine, suggesting that a pattern of PDL against CSPG in the background would be optimal. The results are being applied to a culture system of hippocampal neurons.

Original languageEnglish (US)
Pages (from-to)311-312
Number of pages2
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Issue number1
StatePublished - 1996
EventProceedings of the 1996 18th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 2 (of 5) - Amsterdam, Neth
Duration: Oct 31 1996Nov 3 1996

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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