Microfluidic platforms for on-chip formulation and small-angle X-ray analysis of the phase behavior of lipid/water mixtures

Daria S. Khvostichenko, Sarah L. Perry, Elena Kondrashkina, Sudipto Guha, Keith Brister, Paul J A Kenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a microfluidic platform for on-chip formulation and X-ray analysis of lipidic mesophases formed upon mixing lipids and water. The platform is designed to study the effect of detergents on the phase behavior of lipid/water mixtures. The platform allows automated preparation of multiple samples of different composition from stock solutions and subsequent on-chip small-angle X-ray diffraction (SAXS) data collection. To ensure X-ray transparency of the platform we used thin layers of cyclic olefin copolymer (COC) and PDMS. The viability of the platform is demonstrated by mapping out a section of the phase diagram for lipid monoolein mixed with solutions of detergent β-octylglucoside.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1403-1405
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

X ray analysis
Phase behavior
Microfluidics
Lipids
Detergents
Water
Transparency
Olefins
Phase diagrams
Copolymers
X ray diffraction
X rays
Chemical analysis

Keywords

  • Microfluidics
  • Monoolein
  • Phase diagrams
  • SAXS
  • X-ray transparent chips

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Khvostichenko, D. S., Perry, S. L., Kondrashkina, E., Guha, S., Brister, K., & Kenis, P. J. A. (2011). Microfluidic platforms for on-chip formulation and small-angle X-ray analysis of the phase behavior of lipid/water mixtures. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1403-1405). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

Microfluidic platforms for on-chip formulation and small-angle X-ray analysis of the phase behavior of lipid/water mixtures. / Khvostichenko, Daria S.; Perry, Sarah L.; Kondrashkina, Elena; Guha, Sudipto; Brister, Keith; Kenis, Paul J A.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1403-1405 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Khvostichenko, DS, Perry, SL, Kondrashkina, E, Guha, S, Brister, K & Kenis, PJA 2011, Microfluidic platforms for on-chip formulation and small-angle X-ray analysis of the phase behavior of lipid/water mixtures. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 2, pp. 1403-1405, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.
Khvostichenko DS, Perry SL, Kondrashkina E, Guha S, Brister K, Kenis PJA. Microfluidic platforms for on-chip formulation and small-angle X-ray analysis of the phase behavior of lipid/water mixtures. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1403-1405. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
Khvostichenko, Daria S. ; Perry, Sarah L. ; Kondrashkina, Elena ; Guha, Sudipto ; Brister, Keith ; Kenis, Paul J A. / Microfluidic platforms for on-chip formulation and small-angle X-ray analysis of the phase behavior of lipid/water mixtures. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 1403-1405 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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