TY - GEN
T1 - Microfluidic platform for rapid antibiotic susceptibility testing of polymicrobial communities
AU - Mohan, R.
AU - Sanpitakseree, C.
AU - Sevgen, E.
AU - Desai, A. V.
AU - Schroeder, C. M.
AU - Kenis, P. J.A.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - This paper reports a microfluidic approach for antibiotic susceptibility testing of polymicrobial communities, involving genetically engineered Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. These microbes are associated with clinically significant infections such as cystic fibrosis, urinary tract infections, bacteremia, and chronic wounds. The microfluidic platform allows quantitative measurement of bacterial interaction with single cell resolution while also enabling direct visualization of changes in cell morphology and behavior over extended periods of time. Furthermore, the microfluidic technology was also tested to screen 'real' samples, that is non-genetically engineered bacteria, against commonly used antibiotics such as ampicillin and tobramycin.
AB - This paper reports a microfluidic approach for antibiotic susceptibility testing of polymicrobial communities, involving genetically engineered Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. These microbes are associated with clinically significant infections such as cystic fibrosis, urinary tract infections, bacteremia, and chronic wounds. The microfluidic platform allows quantitative measurement of bacterial interaction with single cell resolution while also enabling direct visualization of changes in cell morphology and behavior over extended periods of time. Furthermore, the microfluidic technology was also tested to screen 'real' samples, that is non-genetically engineered bacteria, against commonly used antibiotics such as ampicillin and tobramycin.
KW - Antibiotic susceptibility testing
KW - Polymicrobial
KW - Time lapse fluorescence microscopy
UR - http://www.scopus.com/inward/record.url?scp=84907375655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907375655&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907375655
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 1353
EP - 1355
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -