Microfluidic platform for rapid antibiotic susceptibility testing of polymicrobial communities

R. Mohan, C. Sanpitakseree, E. Sevgen, A. V. Desai, Charles M Schroeder, Paul J A Kenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a microfluidic approach for antibiotic susceptibility testing of polymicrobial communities, involving genetically engineered Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. These microbes are associated with clinically significant infections such as cystic fibrosis, urinary tract infections, bacteremia, and chronic wounds. The microfluidic platform allows quantitative measurement of bacterial interaction with single cell resolution while also enabling direct visualization of changes in cell morphology and behavior over extended periods of time. Furthermore, the microfluidic technology was also tested to screen 'real' samples, that is non-genetically engineered bacteria, against commonly used antibiotics such as ampicillin and tobramycin.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1353-1355
Number of pages3
ISBN (Print)9781632666246
StatePublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Antibiotic susceptibility testing
  • Polymicrobial
  • Time lapse fluorescence microscopy

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Mohan, R., Sanpitakseree, C., Sevgen, E., Desai, A. V., Schroeder, C. M., & Kenis, P. J. A. (2013). Microfluidic platform for rapid antibiotic susceptibility testing of polymicrobial communities. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1353-1355). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.