Microfluidic patterning of nanodisc lipid bilayers and multiplexed analysis of protein interaction

Edgar D. Goluch, Andrew W. Shaw, Stephen G. Sligar, Chang Liu

Research output: Contribution to journalArticle

Abstract

We report a microfluidic method for precisely patterning lipid bilayers and a multiplexed assay to examine the interaction between the lipids and protein analytes. The lipids were packaged into nanoscale lipid bilayer particles known as Nanodiscs and delivered to surfaces using microfluidic channels. Two types of lipids were used in this study: biontinylated lipids and phosphoserine lipids. The deposition of biotinylated lipids on a glass surface was confirmed by attaching streptavidin coated quantum dots to the lipids, followed by fluorescent imaging. Using this multiplexed grid assay, we examined binding of annexin to phosphoserine lipids, and compared these results to similar analysis performed by surface plasmon resonance.

Original languageEnglish (US)
Pages (from-to)1723-1728
Number of pages6
JournalLab on a chip
Volume8
Issue number10
DOIs
StatePublished - Jan 1 2008

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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