Microfluidic methods in single cell biology

Arnab Mukherjee, Charles M Schroeder

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Stochastic variations within seemingly homogeneous cell populations determine the emergent properties of complex cellular systems such as biofilms, tumors, pluripotent stem cells, and multispecies ecosystems. The advent of microfluidic technologies, coupled with rapid advances in fluorescence-based molecular imaging and genomic, transcriptomic, and proteomic profiling techniques, has spurred a revolution in biological analysis at the level of single cells. Over the past decade, several microfluidic platforms have been developed that enable the isolation, enrichment, and biochemical or genetic analysis of individual cells with high spatiotemporal resolution in a fashion that is not achievable using macroscale methods. In sharp contrast to population-averaged measurements based on bulk-level techniques, microfluidic cell culture platforms permit the acquisition of multiparametric and high-content information while preserving the identity and monitoring the behavior of individual cells over time. In this way, microfluidics has ushered in new frontiers in single cell biology with a direct impact on applied and foundational studies in microbial ecology, systems biology, therapeutics development, and clinical diagnostics. In this chapter, we describe the transformative impact of microfluidics in single cell biology with particular emphasis on the following areas: (1) microfluidic bioreactors for cellular analysis in dynamically changing microenvironments, (2) microfluidic chips for in vitro drug screening, and (3) single cell confinement and isolation microchips for sorting and profiling rare or unculturable cells in complex environmental consortia.

Original languageEnglish (US)
Title of host publicationMicrofluidic Methods for Molecular Biology
PublisherSpringer International Publishing
Pages19-54
Number of pages36
ISBN (Electronic)9783319300191
ISBN (Print)9783319300177
DOIs
StatePublished - Jan 1 2016

Fingerprint

Cytology
Microfluidics
Cell Biology
Cells
Molecular imaging
Pluripotent Stem Cells
Preclinical Drug Evaluations
Molecular Imaging
Systems Biology
Neoplastic Stem Cells
Cell Separation
Biofilms
Bioreactors
Ecology
Stem cells
Sorting
Cell culture
Proteomics
Ecosystems
Population

Keywords

  • Antibiotic resistance
  • Biological noise
  • Circulating tumor cells
  • Laminar flow
  • Single cell genome amplification
  • Stochasticity
  • Time-lapse fluorescent microscopy
  • Unculturable microbes

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

Mukherjee, A., & Schroeder, C. M. (2016). Microfluidic methods in single cell biology. In Microfluidic Methods for Molecular Biology (pp. 19-54). Springer International Publishing. https://doi.org/10.1007/978-3-319-30019-1_2

Microfluidic methods in single cell biology. / Mukherjee, Arnab; Schroeder, Charles M.

Microfluidic Methods for Molecular Biology. Springer International Publishing, 2016. p. 19-54.

Research output: Chapter in Book/Report/Conference proceedingChapter

Mukherjee, A & Schroeder, CM 2016, Microfluidic methods in single cell biology. in Microfluidic Methods for Molecular Biology. Springer International Publishing, pp. 19-54. https://doi.org/10.1007/978-3-319-30019-1_2
Mukherjee A, Schroeder CM. Microfluidic methods in single cell biology. In Microfluidic Methods for Molecular Biology. Springer International Publishing. 2016. p. 19-54 https://doi.org/10.1007/978-3-319-30019-1_2
Mukherjee, Arnab ; Schroeder, Charles M. / Microfluidic methods in single cell biology. Microfluidic Methods for Molecular Biology. Springer International Publishing, 2016. pp. 19-54
@inbook{2b43c1e095754004b39f23f75f398e3b,
title = "Microfluidic methods in single cell biology",
abstract = "Stochastic variations within seemingly homogeneous cell populations determine the emergent properties of complex cellular systems such as biofilms, tumors, pluripotent stem cells, and multispecies ecosystems. The advent of microfluidic technologies, coupled with rapid advances in fluorescence-based molecular imaging and genomic, transcriptomic, and proteomic profiling techniques, has spurred a revolution in biological analysis at the level of single cells. Over the past decade, several microfluidic platforms have been developed that enable the isolation, enrichment, and biochemical or genetic analysis of individual cells with high spatiotemporal resolution in a fashion that is not achievable using macroscale methods. In sharp contrast to population-averaged measurements based on bulk-level techniques, microfluidic cell culture platforms permit the acquisition of multiparametric and high-content information while preserving the identity and monitoring the behavior of individual cells over time. In this way, microfluidics has ushered in new frontiers in single cell biology with a direct impact on applied and foundational studies in microbial ecology, systems biology, therapeutics development, and clinical diagnostics. In this chapter, we describe the transformative impact of microfluidics in single cell biology with particular emphasis on the following areas: (1) microfluidic bioreactors for cellular analysis in dynamically changing microenvironments, (2) microfluidic chips for in vitro drug screening, and (3) single cell confinement and isolation microchips for sorting and profiling rare or unculturable cells in complex environmental consortia.",
keywords = "Antibiotic resistance, Biological noise, Circulating tumor cells, Laminar flow, Single cell genome amplification, Stochasticity, Time-lapse fluorescent microscopy, Unculturable microbes",
author = "Arnab Mukherjee and Schroeder, {Charles M}",
year = "2016",
month = "1",
day = "1",
doi = "10.1007/978-3-319-30019-1_2",
language = "English (US)",
isbn = "9783319300177",
pages = "19--54",
booktitle = "Microfluidic Methods for Molecular Biology",
publisher = "Springer International Publishing",

}

TY - CHAP

T1 - Microfluidic methods in single cell biology

AU - Mukherjee, Arnab

AU - Schroeder, Charles M

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Stochastic variations within seemingly homogeneous cell populations determine the emergent properties of complex cellular systems such as biofilms, tumors, pluripotent stem cells, and multispecies ecosystems. The advent of microfluidic technologies, coupled with rapid advances in fluorescence-based molecular imaging and genomic, transcriptomic, and proteomic profiling techniques, has spurred a revolution in biological analysis at the level of single cells. Over the past decade, several microfluidic platforms have been developed that enable the isolation, enrichment, and biochemical or genetic analysis of individual cells with high spatiotemporal resolution in a fashion that is not achievable using macroscale methods. In sharp contrast to population-averaged measurements based on bulk-level techniques, microfluidic cell culture platforms permit the acquisition of multiparametric and high-content information while preserving the identity and monitoring the behavior of individual cells over time. In this way, microfluidics has ushered in new frontiers in single cell biology with a direct impact on applied and foundational studies in microbial ecology, systems biology, therapeutics development, and clinical diagnostics. In this chapter, we describe the transformative impact of microfluidics in single cell biology with particular emphasis on the following areas: (1) microfluidic bioreactors for cellular analysis in dynamically changing microenvironments, (2) microfluidic chips for in vitro drug screening, and (3) single cell confinement and isolation microchips for sorting and profiling rare or unculturable cells in complex environmental consortia.

AB - Stochastic variations within seemingly homogeneous cell populations determine the emergent properties of complex cellular systems such as biofilms, tumors, pluripotent stem cells, and multispecies ecosystems. The advent of microfluidic technologies, coupled with rapid advances in fluorescence-based molecular imaging and genomic, transcriptomic, and proteomic profiling techniques, has spurred a revolution in biological analysis at the level of single cells. Over the past decade, several microfluidic platforms have been developed that enable the isolation, enrichment, and biochemical or genetic analysis of individual cells with high spatiotemporal resolution in a fashion that is not achievable using macroscale methods. In sharp contrast to population-averaged measurements based on bulk-level techniques, microfluidic cell culture platforms permit the acquisition of multiparametric and high-content information while preserving the identity and monitoring the behavior of individual cells over time. In this way, microfluidics has ushered in new frontiers in single cell biology with a direct impact on applied and foundational studies in microbial ecology, systems biology, therapeutics development, and clinical diagnostics. In this chapter, we describe the transformative impact of microfluidics in single cell biology with particular emphasis on the following areas: (1) microfluidic bioreactors for cellular analysis in dynamically changing microenvironments, (2) microfluidic chips for in vitro drug screening, and (3) single cell confinement and isolation microchips for sorting and profiling rare or unculturable cells in complex environmental consortia.

KW - Antibiotic resistance

KW - Biological noise

KW - Circulating tumor cells

KW - Laminar flow

KW - Single cell genome amplification

KW - Stochasticity

KW - Time-lapse fluorescent microscopy

KW - Unculturable microbes

UR - http://www.scopus.com/inward/record.url?scp=85013361172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85013361172&partnerID=8YFLogxK

U2 - 10.1007/978-3-319-30019-1_2

DO - 10.1007/978-3-319-30019-1_2

M3 - Chapter

AN - SCOPUS:85013361172

SN - 9783319300177

SP - 19

EP - 54

BT - Microfluidic Methods for Molecular Biology

PB - Springer International Publishing

ER -