Microfluidic generation of lipidic mesophases for membrane protein crystallization

Sarah L. Perry, Griffin W. Roberts, Joshua D. Tice, Robert B. Gennis, Paul J.A. Kenis

Research output: Contribution to journalArticlepeer-review


We report on a microfluidic method for the formation of aqueous/lipid mesophases to enable screening of suitable crystallization conditions of membrane proteins from a membrane-like phase in sub-20 nL volumes. This integrated microfluidic chip and the employed mixing strategy address the specific challenges associated with the mixing of fluids of highly different viscosities (here a factor of 30) as well as the non-Newtonian character of the resulting mesophases. The chip requires less than 20 nL of material per condition screened, whereas typically on the order of 10 μL or more is needed for a batch preparation in the present screening methods. We validated our approach with the successful crystallization of the membrane protein bacteriorhodopsin.

Original languageEnglish (US)
Pages (from-to)2566-2569
Number of pages4
JournalCrystal Growth and Design
Issue number6
StatePublished - Jun 3 2009

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science
  • Condensed Matter Physics


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