Microfluidic flow-flash: Method for investigating protein dynamics

Michael W. Toepke, Scott H. Brewer, Dung M. Vu, Kirk D. Rector, Joel E. Morgan, Robert B. Gennis, Paul J.A. Kenis, R. Brian Dyer

Research output: Contribution to journalArticlepeer-review


We report a new method, microfluidic flow-flash, for measuring protein reaction kinetics. The method couples a microscope imaging detection system with a microfluidic flow cell to reduce data acquisition times and sample consumption. This combination allows for the simultaneous collection of spectral and temporal information. The microfluidic flow cell design utilizes three-dimensional sheath flow to reduce sample dispersion and minimize sample consumption. The ability to alter the flow rates in the microfluidic flow cells allows a variety of time scales to be studied with submillisecond time resolution. The imaging detection system can be coupled with several spectroscopic probes including fluorescence and UV/visible absorbance spectroscopy. Here, we utilize the microfluidic flow-flash method to probe the kinetics of CO recombination or O2 binding to myoglobin after the laser-induced photolysis of CO from myoglobin by UV/visible absorbance spectral imaging.

Original languageEnglish (US)
Pages (from-to)122-128
Number of pages7
JournalAnalytical Chemistry
Issue number1
StatePublished - Jan 1 2007

ASJC Scopus subject areas

  • Analytical Chemistry


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