Microfluidic biochip for impedance spectroscopy of biological species

R. Go´mez; R. Bashir; A. Sarikaya; M. R. Ladisch; J. Sturgis; J. P. Robinson; T. Geng; A. K. Bhunia; H. L. Apple; S. Wereley

doi:10.1023/A:1011403112850

Microfluidic biochip for impedance spectroscopy of biological species

R. Go´mez, R. Bashir, A. Sarikaya, M. R. Ladisch, J. Sturgis, J. P. Robinson, T. Geng, A. K. Bhunia, H. L. Apple, S. Wereley

Research output: Contribution to journal › Article › peer-review

Abstract

This paper describes the fabrication and characterization of a microelectronic device for the electrical interrogation and impedance spectroscopy of biological species. Key features of the device include an all top-side processing for the formation of fluidic channels, planar fluidic interface ports, integrated metal electrodes for impedance measurements, and a glass cover sealing the nonplanar topography of the chip using spin-on-glass as an intermediate bonding layer. The total fluidic path volume in the device is on the order of 30 nl. Flow fields in the closed chip were mapped by particle image velocimetry. Electrical impedance measurements of suspensions of the live microorganism Listeria innocua injected into the chip demonstrate an easy method for detecting the viability of a few bacterial cells. By-products of the bacterial metabolism modify the ionic strength of a low conductivity suspension medium, significantly altering its electrical characteristics.

Original language	English (US)
Pages (from-to)	201-209
Number of pages	9
Journal	Biomedical microdevices
Volume	3
Issue number	3
DOIs	https://doi.org/10.1023/A:1011403112850
State	Published - 2001
Externally published	Yes

Keywords

Biochip
Impedance
Listeria
Microfluidic

ASJC Scopus subject areas

Genetics
Medicine (miscellaneous)
General Neuroscience

Online availability

10.1023/A:1011403112850

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title = "Microfluidic biochip for impedance spectroscopy of biological species",

abstract = "This paper describes the fabrication and characterization of a microelectronic device for the electrical interrogation and impedance spectroscopy of biological species. Key features of the device include an all top-side processing for the formation of fluidic channels, planar fluidic interface ports, integrated metal electrodes for impedance measurements, and a glass cover sealing the nonplanar topography of the chip using spin-on-glass as an intermediate bonding layer. The total fluidic path volume in the device is on the order of 30 nl. Flow fields in the closed chip were mapped by particle image velocimetry. Electrical impedance measurements of suspensions of the live microorganism Listeria innocua injected into the chip demonstrate an easy method for detecting the viability of a few bacterial cells. By-products of the bacterial metabolism modify the ionic strength of a low conductivity suspension medium, significantly altering its electrical characteristics.",

keywords = "Biochip, Impedance, Listeria, Microfluidic",

author = "R. Go´mez and R. Bashir and A. Sarikaya and Ladisch, {M. R.} and J. Sturgis and Robinson, {J. P.} and T. Geng and Bhunia, {A. K.} and Apple, {H. L.} and S. Wereley",

note = "Funding Information: This work was supported by a USDA cooperative agreement: CRIS number 1935-42000-035-00D, agreement # 58-1935-9-010. Rafael G{\'o}mez' work is partially funded through a NSF IGERT graduate student fellowship. We thank Dr. John Denton, D. Lubelski, M. Young, and T. Miller of the microelectronics fabrication facility at Purdue University, where the chip fabrication was done. We also thank J. Comeau and M. Skvarla of the NSF-funded Cornell Nanofabrication Facility for the deep reactive ion etch performed on the chip.",

year = "2001",

doi = "10.1023/A:1011403112850",

language = "English (US)",

volume = "3",

pages = "201--209",

journal = "Biomedical microdevices",

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AU - Go´mez, R.

AU - Bashir, R.

AU - Sarikaya, A.

AU - Ladisch, M. R.

AU - Sturgis, J.

AU - Robinson, J. P.

AU - Geng, T.

AU - Bhunia, A. K.

AU - Apple, H. L.

AU - Wereley, S.

N1 - Funding Information: This work was supported by a USDA cooperative agreement: CRIS number 1935-42000-035-00D, agreement # 58-1935-9-010. Rafael Gómez' work is partially funded through a NSF IGERT graduate student fellowship. We thank Dr. John Denton, D. Lubelski, M. Young, and T. Miller of the microelectronics fabrication facility at Purdue University, where the chip fabrication was done. We also thank J. Comeau and M. Skvarla of the NSF-funded Cornell Nanofabrication Facility for the deep reactive ion etch performed on the chip.

PY - 2001

Y1 - 2001

N2 - This paper describes the fabrication and characterization of a microelectronic device for the electrical interrogation and impedance spectroscopy of biological species. Key features of the device include an all top-side processing for the formation of fluidic channels, planar fluidic interface ports, integrated metal electrodes for impedance measurements, and a glass cover sealing the nonplanar topography of the chip using spin-on-glass as an intermediate bonding layer. The total fluidic path volume in the device is on the order of 30 nl. Flow fields in the closed chip were mapped by particle image velocimetry. Electrical impedance measurements of suspensions of the live microorganism Listeria innocua injected into the chip demonstrate an easy method for detecting the viability of a few bacterial cells. By-products of the bacterial metabolism modify the ionic strength of a low conductivity suspension medium, significantly altering its electrical characteristics.

AB - This paper describes the fabrication and characterization of a microelectronic device for the electrical interrogation and impedance spectroscopy of biological species. Key features of the device include an all top-side processing for the formation of fluidic channels, planar fluidic interface ports, integrated metal electrodes for impedance measurements, and a glass cover sealing the nonplanar topography of the chip using spin-on-glass as an intermediate bonding layer. The total fluidic path volume in the device is on the order of 30 nl. Flow fields in the closed chip were mapped by particle image velocimetry. Electrical impedance measurements of suspensions of the live microorganism Listeria innocua injected into the chip demonstrate an easy method for detecting the viability of a few bacterial cells. By-products of the bacterial metabolism modify the ionic strength of a low conductivity suspension medium, significantly altering its electrical characteristics.

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Microfluidic biochip for impedance spectroscopy of biological species

Abstract

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