Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography

Kan Liu, Eric J. Lepin, Ming Wei Wang, Feng Guo, Wei Yu Lin, Yi Chun Chen, Shannon J. Sirk, Sebastian Olma, Michael E. Phelps, Xing Zhong Zhao, Hsian Rong Tseng, R. Michael Van Dam, Anna M. Wu, Clifton K.F. Shen

Research output: Contribution to journalArticlepeer-review

Abstract

Methods for tagging biomolecules with fluorine 18 as immuno-positron emission tomography (immunoPET) tracers require tedious optimization of radiolabeling conditions and can consume large amounts of scarce biomolecules. We describe an improved method using a digital microfluidic droplet generation (DMDG) chip, which provides computer-controlled metering and mixing of 18F tag, biomolecule, and buffer in defined ratios, allowing rapid scouting of reaction conditions in nanoliter volumes. The identified optimized conditions were then translated to bench-scale 18F labeling of a cancer-specific engineered antibody fragments, enabling microPET imaging of tumors in xenografted mice at 0.5 to 4 hours postinjection.

Original languageEnglish (US)
Pages (from-to)168-176
Number of pages9
JournalMolecular imaging
Volume10
Issue number3
DOIs
StatePublished - Jun 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Fingerprint

Dive into the research topics of 'Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography'. Together they form a unique fingerprint.

Cite this