Microencapsulation of docosahexaenoic acid (DHA) with four wall materials including pea protein-modified starch complex

Gulcin Yildiz, Junzhou Ding, Shashank Gaur, Juan Andrade, Nicki E. Engeseth, Hao Feng

Research output: Contribution to journalArticlepeer-review


Omega-3 fatty acids, specifically docosahexaenoic acid (DHA, 22 carbons and 6 double bonds) are fundamental compounds for a healthy diet. However, due to their unsaturated nature, omega fatty acid-rich oils are chemically unstable and susceptible to oxidative deterioration. The oxidation results in production of free radicals and unpleasant tastes, negatively impacting the shelf-life, sensory properties, and acceptability of food products. This study was conducted to examine the effect of wall materials on protection of DHA in canola oil against oxidation. A total of 4 wall materials including pea protein isolate (PPI), pea protein isolate - modified starch complex (PPI-MS), Tween 20, and SDS were used for microemulsion preparation with canola oil containing DHA. The freeze-dried powders were analyzed with respect to physicochemical characteristics, oxidative stability, and release properties. The results showed that the PPI-MS as a natural polymeric wall material exhibited similar or better encapsulation efficiency and acceptable level of peroxide value compared to the synthetic surfactants (Tween 20 and SDS). The utilization of protein-polysaccharide complexes enabled the incorporation of specific properties of each biopolymer to further improve emulsion stability for the production of capsules with improved oxidative stability.

Original languageEnglish (US)
Pages (from-to)935-941
Number of pages7
JournalInternational Journal of Biological Macromolecules
StatePublished - Jul 15 2018


  • Encapsulation efficiency
  • Mano-thermo-sonication (MTS)
  • Omega-3 fatty acid
  • Oxidative stability
  • PPI-modified starch complexes
  • Release property

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology
  • Biochemistry


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