MicroED in natural product and small molecule research

Emma Danelius; Steve Halaby; Wilfred A van der Donk; Tamir Gonen

doi:10.1039/d0np00035c

MicroED in natural product and small molecule research

Emma Danelius, Steve Halaby, Wilfred A van der Donk, Tamir Gonen

Research output: Contribution to journal › Article › peer-review

Abstract

Covering: 2013 to 2020. The electron cryo-microscopy (cryo-EM) method Microcrystal Electron Diffraction (MicroED) allows the collection of high-resolution structural data from vanishingly small crystals that appear like amorphous powders or very fine needles. Since its debut in 2013, data collection and analysis schemes have been fine-tuned, and there are currently close to 100 structures determined by MicroED. Although originally developed to study proteins, MicroED is also very powerful for smaller systems, with some recent and very promising examples from the field of natural products. Herein, we review what has been achieved so far and provide examples of natural product structures, as well as demonstrate the expected future impact of MicroED to the field of natural product and small molecule research.

Original language	English (US)
Pages (from-to)	423-431
Number of pages	9
Journal	Natural Product Reports
Volume	38
Issue number	3
DOIs	https://doi.org/10.1039/d0np00035c
State	Published - Mar 2021

ASJC Scopus subject areas

Drug Discovery
Biochemistry
Organic Chemistry

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10.1039/d0np00035c

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title = "MicroED in natural product and small molecule research",

abstract = "Covering: 2013 to 2020. The electron cryo-microscopy (cryo-EM) method Microcrystal Electron Diffraction (MicroED) allows the collection of high-resolution structural data from vanishingly small crystals that appear like amorphous powders or very fine needles. Since its debut in 2013, data collection and analysis schemes have been fine-tuned, and there are currently close to 100 structures determined by MicroED. Although originally developed to study proteins, MicroED is also very powerful for smaller systems, with some recent and very promising examples from the field of natural products. Herein, we review what has been achieved so far and provide examples of natural product structures, as well as demonstrate the expected future impact of MicroED to the field of natural product and small molecule research.",

author = "Emma Danelius and Steve Halaby and {van der Donk}, {Wilfred A} and Tamir Gonen",

note = "Funding Information: The authors would like to thank all of the collaborators, national and international, who contributed over the years to the development of MicroED. We would also like to thank the following sources of funding: The National Institutes of Health P41GM136508 to T. G. and R01GM058822 to W. A. V. The Gonen and van der Donk laboratories are supported by funds from the Howard Hughes Medical Institute. Wenner-Gren Foundations to E. D. Publisher Copyright: {\textcopyright} 2021 The Royal Society of Chemistry.",

year = "2021",

month = mar,

doi = "10.1039/d0np00035c",

language = "English (US)",

volume = "38",

pages = "423--431",

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AU - Danelius, Emma

AU - Halaby, Steve

AU - van der Donk, Wilfred A

AU - Gonen, Tamir

N1 - Funding Information: The authors would like to thank all of the collaborators, national and international, who contributed over the years to the development of MicroED. We would also like to thank the following sources of funding: The National Institutes of Health P41GM136508 to T. G. and R01GM058822 to W. A. V. The Gonen and van der Donk laboratories are supported by funds from the Howard Hughes Medical Institute. Wenner-Gren Foundations to E. D. Publisher Copyright: © 2021 The Royal Society of Chemistry.

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N2 - Covering: 2013 to 2020. The electron cryo-microscopy (cryo-EM) method Microcrystal Electron Diffraction (MicroED) allows the collection of high-resolution structural data from vanishingly small crystals that appear like amorphous powders or very fine needles. Since its debut in 2013, data collection and analysis schemes have been fine-tuned, and there are currently close to 100 structures determined by MicroED. Although originally developed to study proteins, MicroED is also very powerful for smaller systems, with some recent and very promising examples from the field of natural products. Herein, we review what has been achieved so far and provide examples of natural product structures, as well as demonstrate the expected future impact of MicroED to the field of natural product and small molecule research.

AB - Covering: 2013 to 2020. The electron cryo-microscopy (cryo-EM) method Microcrystal Electron Diffraction (MicroED) allows the collection of high-resolution structural data from vanishingly small crystals that appear like amorphous powders or very fine needles. Since its debut in 2013, data collection and analysis schemes have been fine-tuned, and there are currently close to 100 structures determined by MicroED. Although originally developed to study proteins, MicroED is also very powerful for smaller systems, with some recent and very promising examples from the field of natural products. Herein, we review what has been achieved so far and provide examples of natural product structures, as well as demonstrate the expected future impact of MicroED to the field of natural product and small molecule research.

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MicroED in natural product and small molecule research

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