TY - JOUR
T1 - Microcin J25 inhibits ubiquinol oxidase activity of purified cytochrome bd-I from Escherichia coli
AU - Galván, Adriana Emilce
AU - Chalón, Miriam Carolina
AU - Ríos Colombo, Natalia Soledad
AU - Schurig-Briccio, Lici Ariane
AU - Sosa-Padilla, Bernardo
AU - Gennis, Robert B.
AU - Bellomio, Augusto
N1 - Funding Information:
This work was funded by grants PICT 4610 from the Agencia Nacional de Promoción Científica y Tecnológica, Argentina (ANPCyT) , PIP 06906 CO from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina and PIUNT D641/1 from the Secretaría de Ciencia, Arte e Innovación Tecnológica (SCAIT) from Universidad Nacional de Tucumán (UNT), Argentina .
PY - 2019/5
Y1 - 2019/5
N2 - Microcin J25 (MccJ25), an antimicrobial peptide, targets the respiratory chain but the exact mechanism by which it does so remains unclear. Here, we reveal that MccJ25 is able to inhibit the enzymatic activity of the isolated cytochrome bd-I from E. coli and induces at the same time production of reactive oxygen species. MccJ25 behaves as a dose-dependent weak inhibitor. Intriguingly, MccJ25 is capable of producing a change in the oxidation state of cytochrome bd-I causing its partial reduction in the presence of cyanide. These effects are specific for cytochrome bd-I, since the peptide is not able to act on purified cytochrome bo 3 .
AB - Microcin J25 (MccJ25), an antimicrobial peptide, targets the respiratory chain but the exact mechanism by which it does so remains unclear. Here, we reveal that MccJ25 is able to inhibit the enzymatic activity of the isolated cytochrome bd-I from E. coli and induces at the same time production of reactive oxygen species. MccJ25 behaves as a dose-dependent weak inhibitor. Intriguingly, MccJ25 is capable of producing a change in the oxidation state of cytochrome bd-I causing its partial reduction in the presence of cyanide. These effects are specific for cytochrome bd-I, since the peptide is not able to act on purified cytochrome bo 3 .
KW - Cytochrome bd-I
KW - ROS
KW - Redox peptide
KW - Respiratory chain
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UR - http://www.scopus.com/inward/citedby.url?scp=85062881814&partnerID=8YFLogxK
U2 - 10.1016/j.biochi.2019.02.007
DO - 10.1016/j.biochi.2019.02.007
M3 - Article
C2 - 30790617
AN - SCOPUS:85062881814
SN - 0300-9084
VL - 160
SP - 141
EP - 147
JO - Biochimie
JF - Biochimie
ER -