Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption

Franck Carbonero; Gerardo M. Nava; Ann C. Benefiel; Eugene Greenberg; H. Rex Gaskins

doi:10.1016/j.mimet.2011.07.014

Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption

Franck Carbonero
, Gerardo M. Nava
, Ann C. Benefiel
, Eugene Greenberg
, H. Rex Gaskins

Research output: Contribution to journal › Article › peer-review

Abstract

Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies.

Original language	English (US)
Pages (from-to)	125-127
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	87
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2011.07.014
State	Published - Oct 2011

Keywords

Bead-beating
DNA extraction
Intestinal mucosa
Quantitative PCR

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

Online availability

10.1016/j.mimet.2011.07.014

Library availability

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title = "Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption",

abstract = "Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies.",

keywords = "Bead-beating, DNA extraction, Intestinal mucosa, Quantitative PCR",

author = "Franck Carbonero and Nava, \{Gerardo M.\} and Benefiel, \{Ann C.\} and Eugene Greenberg and Gaskins, \{H. Rex\}",

note = "This work was supported by a grant from the Carle Foundation as well as a Carle Hospital-University of Illinois Translational Research Program grant. The subjects are thanked for their participation in the study and Carle Foundation Hospital staff for cooperation in obtaining specimens and subject data.",

year = "2011",

month = oct,

doi = "10.1016/j.mimet.2011.07.014",

language = "English (US)",

volume = "87",

pages = "125--127",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

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T1 - Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption

AU - Carbonero, Franck

AU - Nava, Gerardo M.

AU - Benefiel, Ann C.

AU - Greenberg, Eugene

AU - Gaskins, H. Rex

N1 - This work was supported by a grant from the Carle Foundation as well as a Carle Hospital-University of Illinois Translational Research Program grant. The subjects are thanked for their participation in the study and Carle Foundation Hospital staff for cooperation in obtaining specimens and subject data.

PY - 2011/10

Y1 - 2011/10

N2 - Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies.

AB - Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies.

KW - Bead-beating

KW - DNA extraction

KW - Intestinal mucosa

KW - Quantitative PCR

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UR - https://www.scopus.com/pages/publications/80052355846#tab=citedBy

U2 - 10.1016/j.mimet.2011.07.014

DO - 10.1016/j.mimet.2011.07.014

M3 - Article

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AN - SCOPUS:80052355846

SN - 0167-7012

VL - 87

SP - 125

EP - 127

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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