Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption

Franck Carbonero, Gerardo M. Nava, Ann C. Benefiel, Eugene Greenberg, H. Rex Gaskins

Research output: Contribution to journalArticle

Abstract

Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies.

Original languageEnglish (US)
Pages (from-to)125-127
Number of pages3
JournalJournal of Microbiological Methods
Volume87
Issue number1
DOIs
StatePublished - Oct 2011

Keywords

  • Bead-beating
  • DNA extraction
  • Intestinal mucosa
  • Quantitative PCR

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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