Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption

Franck Carbonero, Gerardo M. Nava, Ann C. Benefiel, Eugene Greenberg, H. Rex Gaskins

Research output: Contribution to journalArticle

Abstract

Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies.

Original languageEnglish (US)
Pages (from-to)125-127
Number of pages3
JournalJournal of Microbiological Methods
Volume87
Issue number1
DOIs
StatePublished - Oct 1 2011

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Biopsy
DNA

Keywords

  • Bead-beating
  • DNA extraction
  • Intestinal mucosa
  • Quantitative PCR

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption. / Carbonero, Franck; Nava, Gerardo M.; Benefiel, Ann C.; Greenberg, Eugene; Gaskins, H. Rex.

In: Journal of Microbiological Methods, Vol. 87, No. 1, 01.10.2011, p. 125-127.

Research output: Contribution to journalArticle

Carbonero, Franck ; Nava, Gerardo M. ; Benefiel, Ann C. ; Greenberg, Eugene ; Gaskins, H. Rex. / Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption. In: Journal of Microbiological Methods. 2011 ; Vol. 87, No. 1. pp. 125-127.
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