Micro-assembly of functionalized particulate monolayer on C18-derivatized SiO2 surfaces

Tom T. Huang, Tao Geng, Demir Akin, Woo Jin Chang, Jennifer Sturgis, Rashid Bashir, Arun K. Bhunia, J. Paul Robinson, Michael R. Ladisch

Research output: Contribution to journalArticlepeer-review


This work describes a simple approach to immobilize functionalized colloidal microstructures onto a C18-coated SiO2 substrate via specific or non-specific biomediated interactions. Biotinylated bovine serum albumin pre-adsorbed onto a C18 surface was used to mediate the surface assembly of streptavidin-coated microbeads (2.8 μm), while a bare C18 surface was used to immobilize anti-Listeria antibody-coated microbeads (2.8 μm) through hydrophobic interactions. For a C18 surface pre-adsorbed with bovine serum albumin, hydrophobic polystyrene microbeads (0.8 μm) and positively charged dimethylamino microbeads (0.8 μm) were allowed to self-assemble onto the surface. A monolayer with high surface coverage was observed for both polystyrene and dimethylamino microbeads. The adsorption characteristics of Escherichia coli and Listeria monocytogenes on these microbead-based surfaces were studied using fluorescence microscopy. Both streptavidin microbeads preadsorbed with biotinylated anti-Listeria antibody and anti-Listeria antibody-coated microbeads showed specific capture of L. monocytogenes, while polystyrene and dimethylamino microbeads captured both E. coli and L. monocytogenes non-specifically. The preparation of microbead-based surfaces for the construction of microfluidic devices for separation, detection, or analysis of specific biological species is discussed.

Original languageEnglish (US)
Pages (from-to)416-427
Number of pages12
JournalBiotechnology and bioengineering
Issue number4
StatePublished - Aug 20 2003
Externally publishedYes


  • Biotinylated
  • C
  • Self-assembly

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology


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