MHC class II deletion mutant expresses normal levels of transgene encoded class II molecules that have abnormal conformation and impaired antigen presentation ability

S. Ceman, R. Rudersdorf, E. O. Long, R. Demars

Research output: Contribution to journalArticlepeer-review

Abstract

Successive transfers of HLA-DR alpha and beta genes restored expression of HLA-DR antigens to human B-lymphoblastoid cell line, LCL .174, from which all known expressible class II genes are deleted. While transferent cells displayed large amounts of DR on their surfaces, transgene-encoded DR3 molecules lacked a conformation-dependent epitope. DR1-restricted CTL lysis of DR1-expressing transferents pulsed with native influenza virus proteins was greatly reduced; the same cells were efficiently lysed in the presence of CTL-recognized influenza peptides. The properties of DR-expressing transferents of .174 suggest they are defective in producing peptides from exogenous proteins or in forming DR/peptide complexes. Comparison with other DR-expressing deletion mutants indicates that at least one gene in an ~230 kb DNA segment between the DQ1 and Ring 7 loci is needed for normal DR- mediated processing and presentation. Production of DR3 molecules having the conformation-dependent 16.23 epitope and efficient DR1-restricted presentation of influenza viral epitopes occurred in a B cell line that has a mutation specifically eliminating expression of the TAP1 transporter gene, which is in the ~ 230 kb interval and is needed for production of HLA class I/peptide complexes.

Original languageEnglish (US)
Pages (from-to)754-761
Number of pages8
JournalJournal of Immunology
Volume149
Issue number3
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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