Methoxychlor induces atresia by altering Bcl2 factors and inducing caspase activity in mouse ovarian antral follicles in vitro

Mallikarjuna S. Basavarajappa, Bethany N. Karman, Wei Wang, Rupesh K. Gupta, Jodi A. Flaws

Research output: Contribution to journalArticle

Abstract

Methoxychlor (MXC) is an organochlorine pesticide widely used in many countries against various species of insects that attack crops and domestic animals. MXC reduces fertility by increasing atresia (death) of antral follicles in vivo. MXC also induces atresia of antral follicles after 96. h in vitro. The current work tested the hypothesis that MXC induces morphological atresia at early time points (24 and 48. h) by altering pro-apoptotic (Bax, Bok, Casp3, and caspase activity) and anti-apoptotic (Bcl2 and Bcl-xL) factors in the follicles. The results indicate that at 24. h, MXC increased Bcl-xL and Bax mRNA levels and increased the ratio of Bax/. Bcl2. At 48-96. h, MXC induced morphological atresia. At 24-96. h, MXC increased caspase activities. These data suggest that MXC may induce atresia by altering Bcl2 factors and inducing caspase activities in antral follicles.

Original languageEnglish (US)
Pages (from-to)545-551
Number of pages7
JournalReproductive Toxicology
Volume34
Issue number4
DOIs
StatePublished - Dec 1 2012

Keywords

  • Antral follicles
  • Atresia
  • Caspase
  • Methoxychlor
  • Mouse
  • Ovary

ASJC Scopus subject areas

  • Toxicology

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