Methods for examining lens regeneration in xenopus

Research output: Contribution to journalArticle

Abstract

Some vertebrates are able to regenerate the lens following its removal. This includes species in the genus Xenopus (i.e., X. laevis, X. tropicalis, and X. borealis), the only anurans known to undergo lens regeneration. In Xenopus the regenerated lens is derived de novo from cells located within the basal-most layer of the larval corneal epithelium, and is triggered by factors provided by the neural retina. In larval frogs the corneal epithelium is underlain by an endothelium separated from the corneal epithelium except for a small central attachment (i.e., the “stromal-attracting center”). This connection grows larger as the stroma forms and the frogs approach metamorphosis. Here we provide instructions for performing lentectomies (removal of the original lens) to study lens regeneration.

Original languageEnglish (US)
Pages (from-to)268-273
Number of pages6
JournalCold Spring Harbor protocols
Volume2019
Issue number4
DOIs
StatePublished - Apr 2019

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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