Methods for examining lens regeneration in xenopus

Jonathan Joseph Henry, Kimberly J. Perry, Paul W. Hamilton

Research output: Contribution to journalArticle

Abstract

Some vertebrates are able to regenerate the lens following its removal. This includes species in the genus Xenopus (i.e., X. laevis, X. tropicalis, and X. borealis), the only anurans known to undergo lens regeneration. In Xenopus the regenerated lens is derived de novo from cells located within the basal-most layer of the larval corneal epithelium, and is triggered by factors provided by the neural retina. In larval frogs the corneal epithelium is underlain by an endothelium separated from the corneal epithelium except for a small central attachment (i.e., the “stromal-attracting center”). This connection grows larger as the stroma forms and the frogs approach metamorphosis. Here we provide instructions for performing lentectomies (removal of the original lens) to study lens regeneration.

Original languageEnglish (US)
Pages (from-to)268-273
Number of pages6
JournalCold Spring Harbor protocols
Volume2019
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Xenopus
Lenses
Regeneration
Corneal Epithelium
Anura
Endothelium
Vertebrates
Retina

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Methods for examining lens regeneration in xenopus. / Henry, Jonathan Joseph; Perry, Kimberly J.; Hamilton, Paul W.

In: Cold Spring Harbor protocols, Vol. 2019, No. 4, 01.04.2019, p. 268-273.

Research output: Contribution to journalArticle

Henry, Jonathan Joseph ; Perry, Kimberly J. ; Hamilton, Paul W. / Methods for examining lens regeneration in xenopus. In: Cold Spring Harbor protocols. 2019 ; Vol. 2019, No. 4. pp. 268-273.
@article{efa102372a5c49579f2e90e204b9ea90,
title = "Methods for examining lens regeneration in xenopus",
abstract = "Some vertebrates are able to regenerate the lens following its removal. This includes species in the genus Xenopus (i.e., X. laevis, X. tropicalis, and X. borealis), the only anurans known to undergo lens regeneration. In Xenopus the regenerated lens is derived de novo from cells located within the basal-most layer of the larval corneal epithelium, and is triggered by factors provided by the neural retina. In larval frogs the corneal epithelium is underlain by an endothelium separated from the corneal epithelium except for a small central attachment (i.e., the “stromal-attracting center”). This connection grows larger as the stroma forms and the frogs approach metamorphosis. Here we provide instructions for performing lentectomies (removal of the original lens) to study lens regeneration.",
author = "Henry, {Jonathan Joseph} and Perry, {Kimberly J.} and Hamilton, {Paul W.}",
year = "2019",
month = "4",
day = "1",
doi = "10.1101/pdb.prot101527",
language = "English (US)",
volume = "2019",
pages = "268--273",
journal = "Cold Spring Harbor Protocols",
issn = "1559-6095",
publisher = "Cold Spring Harbor Laboratory Press",
number = "4",

}

TY - JOUR

T1 - Methods for examining lens regeneration in xenopus

AU - Henry, Jonathan Joseph

AU - Perry, Kimberly J.

AU - Hamilton, Paul W.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Some vertebrates are able to regenerate the lens following its removal. This includes species in the genus Xenopus (i.e., X. laevis, X. tropicalis, and X. borealis), the only anurans known to undergo lens regeneration. In Xenopus the regenerated lens is derived de novo from cells located within the basal-most layer of the larval corneal epithelium, and is triggered by factors provided by the neural retina. In larval frogs the corneal epithelium is underlain by an endothelium separated from the corneal epithelium except for a small central attachment (i.e., the “stromal-attracting center”). This connection grows larger as the stroma forms and the frogs approach metamorphosis. Here we provide instructions for performing lentectomies (removal of the original lens) to study lens regeneration.

AB - Some vertebrates are able to regenerate the lens following its removal. This includes species in the genus Xenopus (i.e., X. laevis, X. tropicalis, and X. borealis), the only anurans known to undergo lens regeneration. In Xenopus the regenerated lens is derived de novo from cells located within the basal-most layer of the larval corneal epithelium, and is triggered by factors provided by the neural retina. In larval frogs the corneal epithelium is underlain by an endothelium separated from the corneal epithelium except for a small central attachment (i.e., the “stromal-attracting center”). This connection grows larger as the stroma forms and the frogs approach metamorphosis. Here we provide instructions for performing lentectomies (removal of the original lens) to study lens regeneration.

UR - http://www.scopus.com/inward/record.url?scp=85064206364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064206364&partnerID=8YFLogxK

U2 - 10.1101/pdb.prot101527

DO - 10.1101/pdb.prot101527

M3 - Article

VL - 2019

SP - 268

EP - 273

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1559-6095

IS - 4

ER -