Methodology for creating alloplasmic soybean lines by using Glycine tomentella as a maternal parent

R. J. Singh, R. L. Nelson

Research output: Contribution to journalArticlepeer-review


Soybean breeders have not exploited the diversity of the 26 wild perennial species of the subgenus Glycine Willd. that are distantly related to soybean [Glycine max (L.) Merr.] and harbour useful genes. The objective of this study was to develop a methodology for introgressing cytoplasmic and genetic diversity from Glycine tomentella PI 441001 (2n = 78) into cultivated soybean using 'Dwight' (2n = 40) as the male parent. Immature seeds (19-21 days post-pollination) were cultured in vitro to produce F1 plants (2n = 59). Amphidiploid (2n = 118) plants, induced by colchicine treatment, were vigorous and produced mature pods and seeds after backcrossing with 'Dwight'. The BC1 plants (2n = 79) produced mature seeds in crosses with 'Dwight'. Chromosome numbers in BC2F1 plants ranged from 2n = 41-50. From BC2F2 to BC3F1, the number of plants in parentheses with 2n = 40 (275), 2n = 41 (208), 2n = 42 (80), 2n = 43 (27), 2n = 44 (12) and 2n = 45 (3) were identified. Fertile lines were grown in the field during 2012 and 2013. This is the first report of the successful development of new alloplasmic soybean lines with cytoplasm from G. tomentella.

Original languageEnglish (US)
Pages (from-to)624-631
Number of pages8
JournalPlant Breeding
Issue number5
StatePublished - Oct 1 2014


  • Cytoplasmic male sterility
  • Disomic alien addition line
  • Glycine max
  • Immature seed rescue
  • Monosomic alien addition line
  • Tertiary gene pool
  • Wild perennial glycine species

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


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