Method for evaluating the potential of 14C labeled plant polyphenols to cross the blood-brain barrier using accelerator mass spectrometry

Elsa M. Janle, Mary Ann Lila, Michael Grannan, Lauren Wood, Aine Higgins, Gad G. Yousef, Randy B. Rogers, Helen Kim, George S. Jackson, Connie M. Weaver

Research output: Contribution to journalArticle

Abstract

Bioactive compounds in botanicals may be beneficial in preventing age-related neurodegenerative diseases, but for many compounds conventional methods may be inadequate to detect if these compounds cross the blood-brain barrier or to track the pharmacokinetics in the brain. By combining a number of unique technologies it has been possible to utilize the power of AMS to study the pharmacokinetics of bioactive compounds in the brain at very low concentrations. 14C labeled compounds can be biosynthesized by plant cell suspension cultures co-incubated with radioisotopically-labeled sucrose and isolated and separated into a series of bioactive fractions. To study the pharmacokinetics and tissue distribution of 14C labeled plant polyphenols, rats were implanted with jugular catheters, subcutaneous ultrafiltration probes and brain microdialysis probes. Labeled fractions were dosed orally. Interstitial fluid (ISF) and brain microdialysate samples were taken in tandem with blood samples. It was often possible to determine 14C in blood and ISF with a β-counter. However, brain microdialysate samples 14C levels on the order of 107 atoms/sample required AMS technology. The Brain MicrodialysateAUC/SerumAUC ranged from .021- to .029, with the higher values for the glycoside fractions. By using AMS in combination with traditional methods, it is possible to study uptake by blood, distribution to ISF and determine the amount of a dose which can reach the brain and follow the pharmacokinetics in the brain.

Original languageEnglish (US)
Pages (from-to)1313-1316
Number of pages4
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume268
Issue number7-8
DOIs
StatePublished - Apr 1 2010

Keywords

  • 14C labeled plant polyphenols
  • Accelerator mass spectrometry
  • Blood-brain barrier
  • Grape polyphenols
  • Pharmacokinetics

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

Fingerprint Dive into the research topics of 'Method for evaluating the potential of <sup>14</sup>C labeled plant polyphenols to cross the blood-brain barrier using accelerator mass spectrometry'. Together they form a unique fingerprint.

  • Cite this