Method and materials for introducing DNA into Prevotella ruminicola

Mikeljon P. Nikolich (Inventor), Nadja B. Shoemaker (Inventor), Abigail Salyers (Inventor)

Research output: Patent


A method of introducing expressible heterologous DNA into Prevotella ruminicola is provided. The method involves conjugal transfer of a shuttle vector comprising the heterologous DNA operatively linked to a promoter functional in P. ruminicola. The invention also provides shuttle vectors for use in the method and P. ruminicola produced by the method. The invention further provides a tetracycline resistance gene of the TetQ class, or fragments thereof that confer tetracycline resistance, and a protein of the TetQ class that provides resistance to tetracycline by protecting ribosomes from tetracycline, or active fragments thereof. Finally, the invention provides a promoter functional in P. ruminicola and an engineered P. ruminicola comprising expressible foreign DNA.
Original languageEnglish (US)
U.S. patent number5322784
Filing date6/5/91
StatePublished - Jun 21 1994


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