Method and materials for introducing DNA into Prevotella ruminicola

Mikeljon P. Nikolich; Nadja B. Shoemaker; Abigail Salyers

Method and materials for introducing DNA into Prevotella ruminicola

Mikeljon P. Nikolich (Inventor), Nadja B. Shoemaker (Inventor), Abigail Salyers (Inventor)

Research output: Patent

Abstract

A method of introducing expressible heterologous DNA into Prevotella ruminicola is provided. The method involves conjugal transfer of a shuttle vector comprising the heterologous DNA operatively linked to a promoter functional in P. ruminicola. The invention also provides shuttle vectors for use in the method and P. ruminicola produced by the method. The invention further provides a tetracycline resistance gene of the TetQ class, or fragments thereof that confer tetracycline resistance, and a protein of the TetQ class that provides resistance to tetracycline by protecting ribosomes from tetracycline, or active fragments thereof. Finally, the invention provides a promoter functional in P. ruminicola and an engineered P. ruminicola comprising expressible foreign DNA.

Original language	English (US)
U.S. patent number	5322784
Filing date	6/5/91
State	Published - Jun 21 1994

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title = "Method and materials for introducing DNA into Prevotella ruminicola",

abstract = "A method of introducing expressible heterologous DNA into Prevotella ruminicola is provided. The method involves conjugal transfer of a shuttle vector comprising the heterologous DNA operatively linked to a promoter functional in P. ruminicola. The invention also provides shuttle vectors for use in the method and P. ruminicola produced by the method. The invention further provides a tetracycline resistance gene of the TetQ class, or fragments thereof that confer tetracycline resistance, and a protein of the TetQ class that provides resistance to tetracycline by protecting ribosomes from tetracycline, or active fragments thereof. Finally, the invention provides a promoter functional in P. ruminicola and an engineered P. ruminicola comprising expressible foreign DNA.",

author = "Nikolich, {Mikeljon P.} and Shoemaker, {Nadja B.} and Abigail Salyers",

note = "This invention was made with government support provided by Grant No. 59 32U4-7-119 awarded by the United States Department of Agriculture. The government has certain rights in the invention.; 5322784",

year = "1994",

month = jun,

day = "21",

language = "English (US)",

type = "Patent",

}

TY - PAT

T1 - Method and materials for introducing DNA into Prevotella ruminicola

AU - Nikolich, Mikeljon P.

AU - Shoemaker, Nadja B.

AU - Salyers, Abigail

N1 - This invention was made with government support provided by Grant No. 59 32U4-7-119 awarded by the United States Department of Agriculture. The government has certain rights in the invention.

PY - 1994/6/21

Y1 - 1994/6/21

N2 - A method of introducing expressible heterologous DNA into Prevotella ruminicola is provided. The method involves conjugal transfer of a shuttle vector comprising the heterologous DNA operatively linked to a promoter functional in P. ruminicola. The invention also provides shuttle vectors for use in the method and P. ruminicola produced by the method. The invention further provides a tetracycline resistance gene of the TetQ class, or fragments thereof that confer tetracycline resistance, and a protein of the TetQ class that provides resistance to tetracycline by protecting ribosomes from tetracycline, or active fragments thereof. Finally, the invention provides a promoter functional in P. ruminicola and an engineered P. ruminicola comprising expressible foreign DNA.

AB - A method of introducing expressible heterologous DNA into Prevotella ruminicola is provided. The method involves conjugal transfer of a shuttle vector comprising the heterologous DNA operatively linked to a promoter functional in P. ruminicola. The invention also provides shuttle vectors for use in the method and P. ruminicola produced by the method. The invention further provides a tetracycline resistance gene of the TetQ class, or fragments thereof that confer tetracycline resistance, and a protein of the TetQ class that provides resistance to tetracycline by protecting ribosomes from tetracycline, or active fragments thereof. Finally, the invention provides a promoter functional in P. ruminicola and an engineered P. ruminicola comprising expressible foreign DNA.

M3 - Patent

M1 - 5322784

Y2 - 1991/06/05

ER -

Method and materials for introducing DNA into Prevotella ruminicola

Abstract

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