Metals in obex and retropharyngeal lymph nodes of illinois white-tailed deer and their variations associated with CWD status

Nelda A. Rivera, Jan E Novakofski, Hsin Yi Weng, Amy Kelly, Damian Satterthwaite-Phillips, Marilyn Sue O'Hara, Nohra E Mateus-Pinilla

Research output: Contribution to journalArticle

Abstract

Prion proteins (PrPC) are cell membrane glycoproteins that can be found in many cell types, but specially in neurons. Many studies have suggested PrPC‘s participation in metal transport and cellular protection against stress in the central nervous system (CNS). On the other hand PrPSc, the misfolded isoform of PrPC and the pathogenic agent in transmissible spongiform encephalopathies (TSE), has been associated with brain metal dyshomeostasis in prion diseases. Thus, changes in metal concentration associated with protein misfolding and aggregation have been reported for human and animal prion diseases, as well as for other neurodegenerative disorders, such as Parkinson’s and Alzheimer’s disease. The use of metal concentrations in tissues as surrogate markers for early detection of TSEs has been suggested. Studies on the accumulation of metals in free-ranging white-tailed deer have not been conducted. This study established concentrations of copper, iron, manganese, and magnesium in 2 diagnostic tissues used for CWD testing (obex and retropharyngeal lymph nodes (RLN)). We compared these concentrations between tissues and in relation to CWD status. We established reference intervals (RIs) for these metals and explored their ability to discriminate between CWD-positive and CWD-negative animals. Our results indicate that independent of CWD status, white-tailed deer accumulate higher concentrations of Fe, Mn and Mg in RLN than in obex. White-tailed deer infected with CWD accumulated significantly lower concentrations of Mn and Fe than CWD-negative deer. These patterns differed from other species infected with prion diseases. Overlapping values between CWD positive and negative groups indicate that evaluation of these metals in obex and RLN may not be appropriate as a diagnostic tool for CWD infection in white-tailed deer. Because the CWD-negative deer were included in constructing the RIs, high specificities were expected and should be interpreted with caution. Due to the low sensitivity derived from the RIs, we do not recommend using metal concentrations for disease discrimination.

Original languageEnglish (US)
Pages (from-to)48-58
Number of pages11
JournalPrion
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Deer
Lymph Nodes
Metals
Prion Diseases
Prions
Tissue
PrPC Proteins
Animals
Animal Diseases
Aptitude
Membrane Glycoproteins
Neurology
Cell membranes
Manganese
Neurodegenerative Diseases
Magnesium
Neurons
Parkinson Disease
Copper
Brain

Keywords

  • Chronic wasting disease
  • Copper
  • Iron
  • Magnesium
  • Manganese
  • Metals imbalance
  • Prion
  • Transmissible spongiform encephalopathy

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Infectious Diseases

Cite this

Metals in obex and retropharyngeal lymph nodes of illinois white-tailed deer and their variations associated with CWD status. / Rivera, Nelda A.; Novakofski, Jan E; Weng, Hsin Yi; Kelly, Amy; Satterthwaite-Phillips, Damian; O'Hara, Marilyn Sue; Mateus-Pinilla, Nohra E.

In: Prion, Vol. 9, No. 1, 01.01.2015, p. 48-58.

Research output: Contribution to journalArticle

Rivera, Nelda A. ; Novakofski, Jan E ; Weng, Hsin Yi ; Kelly, Amy ; Satterthwaite-Phillips, Damian ; O'Hara, Marilyn Sue ; Mateus-Pinilla, Nohra E. / Metals in obex and retropharyngeal lymph nodes of illinois white-tailed deer and their variations associated with CWD status. In: Prion. 2015 ; Vol. 9, No. 1. pp. 48-58.
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AU - Satterthwaite-Phillips, Damian

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