Metalloprotein Design

Y. Lu, S. Chakraborty, K. D. Miner, T. D. Wilson, A. Mukherjee, Y. Yu, J. Liu, N. M. Marshall

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Metalloproteins catalyze numerous biological reactions ranging from photosynthesis, respiration, nitrogen fixation to signal transduction and complex chemical reactions. It is thus not surprising that metalloproteins account for almost one-half of the total number of proteins in nature. A considerable effort has been directed toward understanding the structure-function relationships using native proteins. However, it is an ultimate challenge to design metalloproteins using only the minimal features required to reproduce their functionalities as well as confer them with novel and unprecedented functionalities learned from the design process. In this chapter, we review some recent successes in the field of metalloprotein design using either de novo designed or native protein scaffolds. Furthermore, metalloprotein design employing unnatural amino acids or non-native cofactor are summarized. Finally, methodologies employing rational design, combinatorial selection, or both methods are also discussed.

Original languageEnglish (US)
Title of host publicationComprehensive Inorganic Chemistry II (Second Edition)
Subtitle of host publicationFrom Elements to Applications
PublisherElsevier Ltd
Pages565-593
Number of pages29
Volume3
ISBN (Print)9780080965291
DOIs
StatePublished - Aug 1 2013

Fingerprint

Metalloproteins
Nitrogen fixation
Signal transduction
Proteins
Photosynthesis
Scaffolds
Chemical reactions
Amino Acids

Keywords

  • Coiled coils
  • Combinatorial protein design
  • Cupredoxins
  • De novo design
  • Electron transfer
  • Expressed protein ligation
  • Helical bundles
  • Heme protein
  • Heme-copper oxidase
  • Myoglobin
  • Native chemical ligation
  • Nitric oxide reductase
  • Non-native cofactors
  • Redesign of proteins
  • Unnatural amino acids
  • Zinc metalloproteins

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Lu, Y., Chakraborty, S., Miner, K. D., Wilson, T. D., Mukherjee, A., Yu, Y., ... Marshall, N. M. (2013). Metalloprotein Design. In Comprehensive Inorganic Chemistry II (Second Edition): From Elements to Applications (Vol. 3, pp. 565-593). Elsevier Ltd. https://doi.org/10.1016/B978-0-08-097774-4.00325-9

Metalloprotein Design. / Lu, Y.; Chakraborty, S.; Miner, K. D.; Wilson, T. D.; Mukherjee, A.; Yu, Y.; Liu, J.; Marshall, N. M.

Comprehensive Inorganic Chemistry II (Second Edition): From Elements to Applications. Vol. 3 Elsevier Ltd, 2013. p. 565-593.

Research output: Chapter in Book/Report/Conference proceedingChapter

Lu, Y, Chakraborty, S, Miner, KD, Wilson, TD, Mukherjee, A, Yu, Y, Liu, J & Marshall, NM 2013, Metalloprotein Design. in Comprehensive Inorganic Chemistry II (Second Edition): From Elements to Applications. vol. 3, Elsevier Ltd, pp. 565-593. https://doi.org/10.1016/B978-0-08-097774-4.00325-9
Lu Y, Chakraborty S, Miner KD, Wilson TD, Mukherjee A, Yu Y et al. Metalloprotein Design. In Comprehensive Inorganic Chemistry II (Second Edition): From Elements to Applications. Vol. 3. Elsevier Ltd. 2013. p. 565-593 https://doi.org/10.1016/B978-0-08-097774-4.00325-9
Lu, Y. ; Chakraborty, S. ; Miner, K. D. ; Wilson, T. D. ; Mukherjee, A. ; Yu, Y. ; Liu, J. ; Marshall, N. M. / Metalloprotein Design. Comprehensive Inorganic Chemistry II (Second Edition): From Elements to Applications. Vol. 3 Elsevier Ltd, 2013. pp. 565-593
@inbook{e47ab08e47f64d2b8f1454ad98d741a3,
title = "Metalloprotein Design",
abstract = "Metalloproteins catalyze numerous biological reactions ranging from photosynthesis, respiration, nitrogen fixation to signal transduction and complex chemical reactions. It is thus not surprising that metalloproteins account for almost one-half of the total number of proteins in nature. A considerable effort has been directed toward understanding the structure-function relationships using native proteins. However, it is an ultimate challenge to design metalloproteins using only the minimal features required to reproduce their functionalities as well as confer them with novel and unprecedented functionalities learned from the design process. In this chapter, we review some recent successes in the field of metalloprotein design using either de novo designed or native protein scaffolds. Furthermore, metalloprotein design employing unnatural amino acids or non-native cofactor are summarized. Finally, methodologies employing rational design, combinatorial selection, or both methods are also discussed.",
keywords = "Coiled coils, Combinatorial protein design, Cupredoxins, De novo design, Electron transfer, Expressed protein ligation, Helical bundles, Heme protein, Heme-copper oxidase, Myoglobin, Native chemical ligation, Nitric oxide reductase, Non-native cofactors, Redesign of proteins, Unnatural amino acids, Zinc metalloproteins",
author = "Y. Lu and S. Chakraborty and Miner, {K. D.} and Wilson, {T. D.} and A. Mukherjee and Y. Yu and J. Liu and Marshall, {N. M.}",
year = "2013",
month = "8",
day = "1",
doi = "10.1016/B978-0-08-097774-4.00325-9",
language = "English (US)",
isbn = "9780080965291",
volume = "3",
pages = "565--593",
booktitle = "Comprehensive Inorganic Chemistry II (Second Edition)",
publisher = "Elsevier Ltd",

}

TY - CHAP

T1 - Metalloprotein Design

AU - Lu, Y.

AU - Chakraborty, S.

AU - Miner, K. D.

AU - Wilson, T. D.

AU - Mukherjee, A.

AU - Yu, Y.

AU - Liu, J.

AU - Marshall, N. M.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Metalloproteins catalyze numerous biological reactions ranging from photosynthesis, respiration, nitrogen fixation to signal transduction and complex chemical reactions. It is thus not surprising that metalloproteins account for almost one-half of the total number of proteins in nature. A considerable effort has been directed toward understanding the structure-function relationships using native proteins. However, it is an ultimate challenge to design metalloproteins using only the minimal features required to reproduce their functionalities as well as confer them with novel and unprecedented functionalities learned from the design process. In this chapter, we review some recent successes in the field of metalloprotein design using either de novo designed or native protein scaffolds. Furthermore, metalloprotein design employing unnatural amino acids or non-native cofactor are summarized. Finally, methodologies employing rational design, combinatorial selection, or both methods are also discussed.

AB - Metalloproteins catalyze numerous biological reactions ranging from photosynthesis, respiration, nitrogen fixation to signal transduction and complex chemical reactions. It is thus not surprising that metalloproteins account for almost one-half of the total number of proteins in nature. A considerable effort has been directed toward understanding the structure-function relationships using native proteins. However, it is an ultimate challenge to design metalloproteins using only the minimal features required to reproduce their functionalities as well as confer them with novel and unprecedented functionalities learned from the design process. In this chapter, we review some recent successes in the field of metalloprotein design using either de novo designed or native protein scaffolds. Furthermore, metalloprotein design employing unnatural amino acids or non-native cofactor are summarized. Finally, methodologies employing rational design, combinatorial selection, or both methods are also discussed.

KW - Coiled coils

KW - Combinatorial protein design

KW - Cupredoxins

KW - De novo design

KW - Electron transfer

KW - Expressed protein ligation

KW - Helical bundles

KW - Heme protein

KW - Heme-copper oxidase

KW - Myoglobin

KW - Native chemical ligation

KW - Nitric oxide reductase

KW - Non-native cofactors

KW - Redesign of proteins

KW - Unnatural amino acids

KW - Zinc metalloproteins

UR - http://www.scopus.com/inward/record.url?scp=84902560439&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902560439&partnerID=8YFLogxK

U2 - 10.1016/B978-0-08-097774-4.00325-9

DO - 10.1016/B978-0-08-097774-4.00325-9

M3 - Chapter

AN - SCOPUS:84902560439

SN - 9780080965291

VL - 3

SP - 565

EP - 593

BT - Comprehensive Inorganic Chemistry II (Second Edition)

PB - Elsevier Ltd

ER -