Metagenomic analysis of microbial communities yields insight into impacts of nanoparticle design

Jacob W. Metch, Nathan D. Burrows, Catherine J. Murphy, Amy Pruden, Peter J. Vikesland

Research output: Contribution to journalArticle

Abstract

Next-generation DNA sequencing and metagenomic analysis provide powerful tools for the environmentally friendly design of nanoparticles. Herein we demonstrate this approach using a model community of environmental microbes (that is, wastewater-activated sludge) dosed with gold nanoparticles of varying surface coatings and morphologies. Metagenomic analysis was highly sensitive in detecting the microbial community response to gold nanospheres and nanorods with either cetyltrimethylammonium bromide or polyacrylic acid surface coatings. We observed that the gold-nanoparticle morphology imposes a stronger force in shaping the microbial community structure than does the surface coating. Trends were consistent in terms of the compositions of both taxonomic and functional genes, which include antibiotic resistance genes, metal resistance genes and gene-transfer elements associated with cell stress that are relevant to public health. Given that nanoparticle morphology remained constant, the potential influence of gold dissolution was minimal. Surface coating governed the nanoparticle partitioning between the bioparticulate and aqueous phases.

Original languageEnglish (US)
Pages (from-to)253-259
Number of pages7
JournalNature Nanotechnology
Volume13
Issue number3
DOIs
StatePublished - Mar 1 2018

Fingerprint

Gold
genes
Nanoparticles
nanoparticles
gold
coatings
Coatings
carbopol 940
Genes
activated sludge
Gene transfer
public health
sequencing
antibiotics
Nanospheres
Public health
Antibiotics
microorganisms
Nanorods
Chemical elements

ASJC Scopus subject areas

  • Bioengineering
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Metagenomic analysis of microbial communities yields insight into impacts of nanoparticle design. / Metch, Jacob W.; Burrows, Nathan D.; Murphy, Catherine J.; Pruden, Amy; Vikesland, Peter J.

In: Nature Nanotechnology, Vol. 13, No. 3, 01.03.2018, p. 253-259.

Research output: Contribution to journalArticle

Metch, Jacob W. ; Burrows, Nathan D. ; Murphy, Catherine J. ; Pruden, Amy ; Vikesland, Peter J. / Metagenomic analysis of microbial communities yields insight into impacts of nanoparticle design. In: Nature Nanotechnology. 2018 ; Vol. 13, No. 3. pp. 253-259.
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