Metabolomes of the psychrotolerant bacterium Listeria monocytogenes 10403S grown at 37°C and 8°C

Atul K. Singh, Alexander V. Ulanov, Zhong Li, Radheshyam K. Jayaswal, Brian J. Wilkinson

Research output: Contribution to journalArticle

Abstract

Listeria monocytogenes is a food-borne pathogen with the ability to grow at refrigeration temperatures. Knowledge of the mechanisms involved in low temperature growth is incomplete and here we report the results of a metabolomics investigation of this. The small molecule contents of L. monocytogenes 10403S grown at 37°C and 8°C were compared by gas chromatography/mass spectrometry (GC/MS). Over 500 peaks were detected in both 37°C and 8°C-grown cells, and 103 were identified. Of the identified metabolites, the concentrations of 56 metabolites were increased (P < 0.05), while the concentrations of 8 metabolites were decreased at low temperature. Metabolites increasing in concentration at 8°C included amino acids, sugars, organic acids, urea cycle intermediates, polyamines, and different compatible solutes. A principal component analysis (PCA) was used to visualize and compare the matrix containing the data in 6 samples, and this clearly identified the 37°C and 8°C metabolomes as different. The results indicated that an increase in solute concentrations in the cytoplasm was associated with low temperature adaptation, which may be a response to chill stress with the effect of lowering the freezing point of intracellular water and decreasing ice crystal formation.

Original languageEnglish (US)
Pages (from-to)107-114
Number of pages8
JournalInternational Journal of Food Microbiology
Volume148
Issue number2
DOIs
StatePublished - Aug 2 2011

Keywords

  • Cold adaptation
  • Compatible solutes
  • Metabolites profiling

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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