TY - JOUR
T1 - Metabolism of the 4-Hydroxyphenylpyruvate Dioxygenase Inhibitor, Mesotrione, in Multiple-Herbicide-Resistant Palmer amaranth (Amaranthus palmeri)
AU - Concepcion, Jeanaflor Crystal T.
AU - Kaundun, Shiv S.
AU - Morris, James A.
AU - Brandenburg, Autumn N.
AU - Riechers, Dean E.
N1 - The authors are thankful to the following researchers: Drs. Peter Yau and Brian Imai of the Roy J. Carver Biotechnology Center Proteomics Core Facility, University of Illinois at Urbana-Champaign (UIUC), for assistance with phosphor-imaging of TLC plates; Drs. Zhong Li, Michael La Frano, and Alexander Ulanov for providing metabolite profiling services at the UIUC Metabolomics Laboratory; Sarah-Jane Hutchings and Will Plumb (Syngenta-Ltd.) for assistance in initial greenhouse screening and bulking up seeds of Palmer amaranth populations; Rosie Metallo, Montgomery Flack, Alison Ross, Noeleen Brown, Molly Newman-Johnson, and Stuart Seputro (UIUC) for their assistance in greenhouse studies and maintenance of plant samples for metabolism experiments. D.E.R. and A.N.B. thank the Weed Science Society of America for supporting this research through the John Jachetta Undergraduate Research Award. The authors also thank Syngenta-Ltd. for providing seeds of weedy Amaranthus, mesotrione, and metabolite standards.
PY - 2024/3/20
Y1 - 2024/3/20
N2 - Metabolic resistance to the maize-selective, HPPD-inhibiting herbicide, mesotrione, occurs via Phase I ring hydroxylation in resistant waterhemp and Palmer amaranth; however, mesotrione detoxification pathways post-Phase I are unknown. This research aims to (1) evaluate Palmer amaranth populations for mesotrione resistance via survivorship, foliar injury, and aboveground biomass, (2) determine mesotrione metabolism rates in Palmer amaranth populations during a time course, and (3) identify mesotrione metabolites including and beyond Phase I oxidation. The Palmer amaranth populations, SYNR1 and SYNR2, exhibited higher survival rates (100%), aboveground biomass (c.a. 50%), and lower injury (25-30%) following mesotrione treatment than other populations studied. These two populations also metabolized mesotrione 2-fold faster than sensitive populations, PPI1 and PPI2, and rapidly formed 4-OH-mesotrione. Additionally, SYNR1 and SYNR2 formed 5-OH-mesotrione, which is not produced in high abundance in waterhemp or naturally tolerant maize. Metabolite features derived from 4/5-OH-mesotrione and potential Phase II mesotrione-conjugates were detected and characterized by liquid chromatography-mass spectrometry (LCMS).
AB - Metabolic resistance to the maize-selective, HPPD-inhibiting herbicide, mesotrione, occurs via Phase I ring hydroxylation in resistant waterhemp and Palmer amaranth; however, mesotrione detoxification pathways post-Phase I are unknown. This research aims to (1) evaluate Palmer amaranth populations for mesotrione resistance via survivorship, foliar injury, and aboveground biomass, (2) determine mesotrione metabolism rates in Palmer amaranth populations during a time course, and (3) identify mesotrione metabolites including and beyond Phase I oxidation. The Palmer amaranth populations, SYNR1 and SYNR2, exhibited higher survival rates (100%), aboveground biomass (c.a. 50%), and lower injury (25-30%) following mesotrione treatment than other populations studied. These two populations also metabolized mesotrione 2-fold faster than sensitive populations, PPI1 and PPI2, and rapidly formed 4-OH-mesotrione. Additionally, SYNR1 and SYNR2 formed 5-OH-mesotrione, which is not produced in high abundance in waterhemp or naturally tolerant maize. Metabolite features derived from 4/5-OH-mesotrione and potential Phase II mesotrione-conjugates were detected and characterized by liquid chromatography-mass spectrometry (LCMS).
KW - cytochrome P450 monooxygenase
KW - dioecious amaranth
KW - herbicide detoxification
KW - mesotrione resistance
KW - metabolomics
KW - oxidative metabolism
KW - waterhemp
UR - https://www.scopus.com/pages/publications/85187142846
UR - https://www.scopus.com/pages/publications/85187142846#tab=citedBy
U2 - 10.1021/acs.jafc.3c06903
DO - 10.1021/acs.jafc.3c06903
M3 - Article
C2 - 38446412
AN - SCOPUS:85187142846
SN - 0021-8561
VL - 72
SP - 5595
EP - 5608
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 11
ER -