Metabolism of the 4-Hydroxyphenylpyruvate Dioxygenase Inhibitor, Mesotrione, in Multiple-Herbicide-Resistant Palmer amaranth (Amaranthus palmeri)

Jeanaflor Crystal T. Concepcion, Shiv S. Kaundun, James A. Morris, Autumn N. Brandenburg, Dean E. Riechers

Research output: Contribution to journalArticlepeer-review

Abstract

Metabolic resistance to the maize-selective, HPPD-inhibiting herbicide, mesotrione, occurs via Phase I ring hydroxylation in resistant waterhemp and Palmer amaranth; however, mesotrione detoxification pathways post-Phase I are unknown. This research aims to (1) evaluate Palmer amaranth populations for mesotrione resistance via survivorship, foliar injury, and aboveground biomass, (2) determine mesotrione metabolism rates in Palmer amaranth populations during a time course, and (3) identify mesotrione metabolites including and beyond Phase I oxidation. The Palmer amaranth populations, SYNR1 and SYNR2, exhibited higher survival rates (100%), aboveground biomass (c.a. 50%), and lower injury (25-30%) following mesotrione treatment than other populations studied. These two populations also metabolized mesotrione 2-fold faster than sensitive populations, PPI1 and PPI2, and rapidly formed 4-OH-mesotrione. Additionally, SYNR1 and SYNR2 formed 5-OH-mesotrione, which is not produced in high abundance in waterhemp or naturally tolerant maize. Metabolite features derived from 4/5-OH-mesotrione and potential Phase II mesotrione-conjugates were detected and characterized by liquid chromatography-mass spectrometry (LCMS).

Original languageEnglish (US)
Pages (from-to)5595-5608
Number of pages14
JournalJournal of Agricultural and Food Chemistry
Volume72
Issue number11
DOIs
StatePublished - Mar 20 2024

Keywords

  • cytochrome P450 monooxygenase
  • dioecious amaranth
  • herbicide detoxification
  • mesotrione resistance
  • metabolomics
  • oxidative metabolism
  • waterhemp

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences

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