Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy

Yong Keun Park, Catherine Best, Thorsten Auth, Nir S. Gov, Samuel Safran, Gabriel Popescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have quantitatively and systemically measured the morphologies and dynamics of fluctuations in human RBC membranes using a full-field laser interferometry technique that accurately measures dynamic membrane fluctuations. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates nonequilibrium dynamic fluctuations in the RBC membrane and that these fluctuations are highly correlated with specific regions in the biconcave shape of RBCs. Spatial analysis reveals that these nonequilibrium membrane fluctuations are enhanced at the scale of the spectrin mesh size. Our results indicate the presence of dynamic remodeling in the RBC membrane cortex powered by ATP, which results in nonequilibrium membrane fluctuations.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
DOIs
StatePublished - Apr 20 2011
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX - San Francisco, CA, United States
Duration: Jan 22 2011Jan 25 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7902
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
CountryUnited States
CitySan Francisco, CA
Period1/22/111/25/11

Fingerprint

erythrocytes
Cell membranes
Microscopy
Microscopic examination
Blood
Erythrocytes
Cell Membrane
membranes
microscopy
Membranes
adenosines
Adenosine
Adenosine Triphosphate
Interferometry
Laser interferometry
Spectrin
laser interferometry
Spatial Analysis
cortexes
mesh

Keywords

  • ATP
  • biophysics
  • membrane dynamics
  • quantitative phase microscopy
  • red blood cell

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Park, Y. K., Best, C., Auth, T., Gov, N. S., Safran, S., & Popescu, G. (2011). Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX [79021M] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7902). https://doi.org/10.1117/12.874768

Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy. / Park, Yong Keun; Best, Catherine; Auth, Thorsten; Gov, Nir S.; Safran, Samuel; Popescu, Gabriel.

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX. 2011. 79021M (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7902).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Park, YK, Best, C, Auth, T, Gov, NS, Safran, S & Popescu, G 2011, Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX., 79021M, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.874768
Park YK, Best C, Auth T, Gov NS, Safran S, Popescu G. Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX. 2011. 79021M. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.874768
Park, Yong Keun ; Best, Catherine ; Auth, Thorsten ; Gov, Nir S. ; Safran, Samuel ; Popescu, Gabriel. / Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy. Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX. 2011. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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