Metabolic engineering of Parageobacillus thermoglucosidasius for the efficient production of (2R, 3R)-butanediol

Jiewen Zhou, Jiazhang Lian, Christopher V. Rao

Research output: Contribution to journalArticlepeer-review


High-temperature fermentation using thermophilic microorganisms may provide cost-effective processes for the industrial production of fuels and chemicals, due to decreased hygiene and cooling costs. In the present study, the genetically trackable thermophile Parageobacillus thermoglucosidasius DSM2542T was engineered to produce (2R, 3R)-butanediol (R-BDO), a valuable chemical with broad industrial applications. The R-BDO biosynthetic pathway was optimized by testing different combinations of pathway enzymes, with acetolactate synthase (AlsS) from Bacillus subtilis and acetolactate decarboxylase (AlsD) from Streptococcus thermophilus yielding the highest production in P. thermoglucosidasius DSM2542T. Following fermentation condition optimization, shake flask fermentation at 55 °C resulted in the production of 7.2 g/L R-BDO with ~ 72% theoretical yield. This study details the microbial production of R-BDO at the highest fermentation temperature reported to date and demonstrates that P. thermoglucosidasius DSM2542T is a promising cell factory for the production of fuels and chemicals using high-temperature fermentation.

Original languageEnglish (US)
Pages (from-to)4303-4311
Number of pages9
JournalApplied Microbiology and Biotechnology
Issue number10
StatePublished - May 1 2020


  • 2, 3-butanediol
  • Metabolic engineering
  • Parageobacillus thermoglucosidasius
  • Thermophile

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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