Metabolic engineering of non-pathogenic microorganisms for 2,3-butanediol production

Jae Won Lee, Ye Gi Lee, Yong Su Jin, Christopher V. Rao

Research output: Contribution to journalReview articlepeer-review


Abstract: 2,3-Butanediol (2,3-BDO) is a promising commodity chemical with various industrial applications. While petroleum-based chemical processes currently dominate the industrial production of 2,3-BDO, fermentation-based production of 2,3-BDO provides an attractive alternative to chemical-based processes with regards to economic and environmental sustainability. The achievement of high 2,3-BDO titer, yield, and productivity in microbial fermentation is a prerequisite for the production of 2,3-BDO at large scales. Also, enantiopure production of 2,3-BDO production is desirable because 2,3-BDO stereoisomers have unique physicochemical properties. Pursuant to these goals, many metabolic engineering strategies to improve 2,3-BDO production from inexpensive sugars by Klebsiella oxytoca, Bacillus species, and Saccharomyces cerevisiae have been developed. This review summarizes the recent advances in metabolic engineering of non-pathogenic microorganisms to enable efficient and enantiopure production of 2,3-BDO. Key points: • K. oxytoca, Bacillus species, and S. cerevisiae have been engineered to achieve efficient 2,3-BDO production. • Metabolic engineering of non-pathogenic microorganisms enabled enantiopure production of 2,3-BDO. • Cost-effective 2,3-BDO production can be feasible by using renewable biomass.

Original languageEnglish (US)
Pages (from-to)5751-5767
Number of pages17
JournalApplied Microbiology and Biotechnology
Issue number14-15
StatePublished - Aug 2021


  • 2,3-BDO stereoisomers
  • 2,3-Butanediol (2,3-BDO)
  • Bacillus species
  • Klebsiella oxytoca
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology


Dive into the research topics of 'Metabolic engineering of non-pathogenic microorganisms for 2,3-butanediol production'. Together they form a unique fingerprint.

Cite this