Metabolic Engineering: Methodologies and Applications

Michael J. Volk, Vinh G. Tran, Shih I. Tan, Shekhar Mishra, Zia Fatma, Aashutosh Boob, Hongxiang Li, Pu Xue, Teresa A. Martin, Huimin Zhao

Research output: Contribution to journalReview articlepeer-review


Metabolic engineering aims to improve the production of economically valuable molecules through the genetic manipulation of microbial metabolism. While the discipline is a little over 30 years old, advancements in metabolic engineering have given way to industrial-level molecule production benefitting multiple industries such as chemical, agriculture, food, pharmaceutical, and energy industries. This review describes the design, build, test, and learn steps necessary for leading a successful metabolic engineering campaign. Moreover, we highlight major applications of metabolic engineering, including synthesizing chemicals and fuels, broadening substrate utilization, and improving host robustness with a focus on specific case studies. Finally, we conclude with a discussion on perspectives and future challenges related to metabolic engineering.

Original languageEnglish (US)
Pages (from-to)5521-5570
Number of pages50
JournalChemical reviews
Issue number9
StatePublished - May 10 2023

ASJC Scopus subject areas

  • General Chemistry


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