TY - JOUR
T1 - Metabolic Engineering
T2 - Methodologies and Applications
AU - Volk, Michael J.
AU - Tran, Vinh G.
AU - Tan, Shih I.
AU - Mishra, Shekhar
AU - Fatma, Zia
AU - Boob, Aashutosh
AU - Li, Hongxiang
AU - Xue, Pu
AU - Martin, Teresa A.
AU - Zhao, Huimin
N1 - Funding Information:
We thank financial support from the U.S. Department of Energy (DE-SC0018420 and DE-SC0018260) for various metabolic engineering projects (H.Z.). The online tool of BioRender ( BioRender.com ) was used to create parts of the TOC Figures 2–18.
Publisher Copyright:
© 2022 American Chemical Society.
PY - 2022
Y1 - 2022
N2 - Metabolic engineering aims to improve the production of economically valuable molecules through the genetic manipulation of microbial metabolism. While the discipline is a little over 30 years old, advancements in metabolic engineering have given way to industrial-level molecule production benefitting multiple industries such as chemical, agriculture, food, pharmaceutical, and energy industries. This review describes the design, build, test, and learn steps necessary for leading a successful metabolic engineering campaign. Moreover, we highlight major applications of metabolic engineering, including synthesizing chemicals and fuels, broadening substrate utilization, and improving host robustness with a focus on specific case studies. Finally, we conclude with a discussion on perspectives and future challenges related to metabolic engineering.
AB - Metabolic engineering aims to improve the production of economically valuable molecules through the genetic manipulation of microbial metabolism. While the discipline is a little over 30 years old, advancements in metabolic engineering have given way to industrial-level molecule production benefitting multiple industries such as chemical, agriculture, food, pharmaceutical, and energy industries. This review describes the design, build, test, and learn steps necessary for leading a successful metabolic engineering campaign. Moreover, we highlight major applications of metabolic engineering, including synthesizing chemicals and fuels, broadening substrate utilization, and improving host robustness with a focus on specific case studies. Finally, we conclude with a discussion on perspectives and future challenges related to metabolic engineering.
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U2 - 10.1021/acs.chemrev.2c00403
DO - 10.1021/acs.chemrev.2c00403
M3 - Review article
C2 - 36584306
AN - SCOPUS:85145468630
SN - 0009-2665
JO - Chemical Reviews
JF - Chemical Reviews
ER -