Merely two mutations switch a DNA-hydrolyzing deoxyribozyme from heterobimetallic (Zn2+/Mn2+) to monometallic (Zn2+-only) behavior

Ying Xiao; Emily C. Allen; Scott K. Silverman

doi:10.1039/c0cc04575f

Merely two mutations switch a DNA-hydrolyzing deoxyribozyme from heterobimetallic (Zn²⁺/Mn²⁺) to monometallic (Zn²⁺-only) behavior

Ying Xiao, Emily C. Allen, Scott K. Silverman

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A deoxyribozyme that hydrolyzes DNA phosphodiester linkages with a requirement for both Zn²⁺ and Mn²⁺ is switched by only two nucleotide mutations to require Zn²⁺ alone, demonstrating that DNA-catalyzed DNA hydrolysis can be achieved using only one metal ion cofactor.

Original language	English (US)
Pages (from-to)	1749-1751
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	6
DOIs	https://doi.org/10.1039/c0cc04575f
State	Published - Jan 27 2011

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Online availability

10.1039/c0cc04575f

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Cite this

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abstract = "A deoxyribozyme that hydrolyzes DNA phosphodiester linkages with a requirement for both Zn2+ and Mn2+ is switched by only two nucleotide mutations to require Zn2+ alone, demonstrating that DNA-catalyzed DNA hydrolysis can be achieved using only one metal ion cofactor.",

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