TY - JOUR
T1 - MEMS based force sensors for the study of indentation response of single living cells
AU - Yang, Shengyuan
AU - Saif, M. Taher A.
N1 - Funding Information:
We thank Prof. N. Wang of Harvard School of Public Health for helpful discussions. This work was supported by the National Science Foundation (NSF) grants ECS 01-18003 and ECS 05-24675. The MEMS force sensors were fabricated in the Center for Nanoscale Science and Technology at the University of Illinois at Urbana-Champaign.
PY - 2007/3/30
Y1 - 2007/3/30
N2 - This paper presents bio-microelectromechanical systems (MEMS) force sensors, consisting of a probe and flexible beams, to study mechanobiological response of single living cells subjected to mechanical injury. Mechanical injuries are simulated by the application of large indentation on the cells by the sensor probe. The green fluorescent protein (GFP) technique is used to visualize the evolution of the actin network in the cells. The lateral indentation force response measurement capability of the sensors is shown. The cell force response is strongly linear for the initial indentation stage and the cells become yielded due to further indentation, which were not observed before. Two new types of remodeling, large buckling of stress fibers and actin agglomeration, in the actin network in a cell due to mechanical stimuli are also reported.
AB - This paper presents bio-microelectromechanical systems (MEMS) force sensors, consisting of a probe and flexible beams, to study mechanobiological response of single living cells subjected to mechanical injury. Mechanical injuries are simulated by the application of large indentation on the cells by the sensor probe. The green fluorescent protein (GFP) technique is used to visualize the evolution of the actin network in the cells. The lateral indentation force response measurement capability of the sensors is shown. The cell force response is strongly linear for the initial indentation stage and the cells become yielded due to further indentation, which were not observed before. Two new types of remodeling, large buckling of stress fibers and actin agglomeration, in the actin network in a cell due to mechanical stimuli are also reported.
KW - Actin fibers
KW - Cell mechanics
KW - Green fluorescent protein (GFP)
KW - Indentation
KW - Microelectromechanical systems (MEMS)
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U2 - 10.1016/j.sna.2006.05.019
DO - 10.1016/j.sna.2006.05.019
M3 - Article
AN - SCOPUS:33947199355
VL - 135
SP - 16
EP - 22
JO - Sensors and Actuators, A: Physical
JF - Sensors and Actuators, A: Physical
SN - 0924-4247
IS - 1
ER -