Meloidogyne javanica chorismate mutase transcript expression profile using real-time quantitative RT-PCR

Janet E. Painter, Kris Nicholas Lambert

Research output: Contribution to journalArticlepeer-review

Abstract

A developmental expression profile of the Meloidodgyne javanica esophageal gland gene chorismate mutase-1 (Mj-cm-1) could suggest when in the lifecycle of the nematode the Mj-cm-1 product is functional. This study used real-time quantitative RT-PCR to examine the variation in Mj-cm-1 transcript levels over six timepoints in the nematode lifecycle: egg, infective second-stage juveniles (Inf-J2), 2-day post-inoculation (pi), 7-day pi, 14-day pi, and adult. The Mj-cm-1 mRNA levels peaked at 2-day pi, about 100-fold above levels expressed at the egg and Inf-J2 stages. Some expression of Mj-cra-1 remained during the 7-day pi, 14-day pi, and adult stages. High transcript levels of the beta-actin control gene M. javanica Beta-actin-1 (Mj-ba-1) demonstrated the presence of cDNA at all timepoints. The peak in Mj-cm-1 transcript expression at 2-day pi as well as the previously shown esophageal gland localization of Mj-cm-1 mRNA suggest that the product of this gene may be involved early in the establishment of parasitism.

Original languageEnglish (US)
Pages (from-to)82-87
Number of pages6
JournalJournal of Nematology
Volume35
Issue number1
StatePublished - Mar 2003

Keywords

  • cDNA
  • Chorismate mutase
  • Developmental profile
  • Meloidogyne javanica
  • Mj-ba-1
  • Mj-cm-1
  • mRNA
  • Nematode
  • Parasitism gene
  • Real-time quantitative RT-PCR
  • Root-knot nematode
  • Shikimate pathway
  • Taqman assay
  • Transcript

ASJC Scopus subject areas

  • Animal Science and Zoology

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