TY - JOUR
T1 - Meiosis-specific transcripts of a DNA component replicated during chromosome pairing
T2 - Homology across the phylogenetic spectrum
AU - Hotta, Yasuo
AU - Tabata, Satoshi
AU - Stubbs, Lisa
AU - Stern, Herbert
N1 - Funding Information:
This research was supported by grants from the National Science Foundation, the American Cancer Society, and the National Institute for Child Health and Human Development. We wish to thank P E. Geiduschek and M. Simon for their helpful reading of the manuscript. The costs of publication of this article were defrayed in part by the
PY - 1985/4
Y1 - 1985/4
N2 - In meiotic cells of Lilium, a group of single or low copy number DNA sequences that constitute about 0.10.2% of the genome do not replicate during the premeiotic S-phase but do so at zygotene in coordination with chromosome pairing. An appreciable fraction of these sequences has now been found to be transcribed into poly(A)+ RNA when chromosomes initiate the pairing process. This "zygRNA" has not been detected in nonmeiotic tissues. Even within the meiocytes, zygRNA is not detectable prior to leptotene or beyond midpachytene. S1 nuclease digestion of mouse spermatocyte nuclei selectively released zygDNA, which hybridizes with lily zygRNA, zygRNA has not been detected in mouse somatic tissues. The profile of zygRNA formation and disappearance in mouse spermatocytes is very similar to that of zygRNA in lily meiocytes.
AB - In meiotic cells of Lilium, a group of single or low copy number DNA sequences that constitute about 0.10.2% of the genome do not replicate during the premeiotic S-phase but do so at zygotene in coordination with chromosome pairing. An appreciable fraction of these sequences has now been found to be transcribed into poly(A)+ RNA when chromosomes initiate the pairing process. This "zygRNA" has not been detected in nonmeiotic tissues. Even within the meiocytes, zygRNA is not detectable prior to leptotene or beyond midpachytene. S1 nuclease digestion of mouse spermatocyte nuclei selectively released zygDNA, which hybridizes with lily zygRNA, zygRNA has not been detected in mouse somatic tissues. The profile of zygRNA formation and disappearance in mouse spermatocytes is very similar to that of zygRNA in lily meiocytes.
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U2 - 10.1016/0092-8674(85)90338-1
DO - 10.1016/0092-8674(85)90338-1
M3 - Article
C2 - 2580636
AN - SCOPUS:0021997320
SN - 0092-8674
VL - 40
SP - 785
EP - 793
JO - Cell
JF - Cell
IS - 4
ER -