Meiosis-specific transcripts of a DNA component replicated during chromosome pairing: Homology across the phylogenetic spectrum

Yasuo Hotta, Satoshi Tabata, Lisa Stubbs, Herbert Stern

Research output: Contribution to journalArticlepeer-review

Abstract

In meiotic cells of Lilium, a group of single or low copy number DNA sequences that constitute about 0.10.2% of the genome do not replicate during the premeiotic S-phase but do so at zygotene in coordination with chromosome pairing. An appreciable fraction of these sequences has now been found to be transcribed into poly(A)+ RNA when chromosomes initiate the pairing process. This "zygRNA" has not been detected in nonmeiotic tissues. Even within the meiocytes, zygRNA is not detectable prior to leptotene or beyond midpachytene. S1 nuclease digestion of mouse spermatocyte nuclei selectively released zygDNA, which hybridizes with lily zygRNA, zygRNA has not been detected in mouse somatic tissues. The profile of zygRNA formation and disappearance in mouse spermatocytes is very similar to that of zygRNA in lily meiocytes.

Original languageEnglish (US)
Pages (from-to)785-793
Number of pages9
JournalCell
Volume40
Issue number4
DOIs
StatePublished - Apr 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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