Mechanistic studies on the flavin:NADH reductase (PrnF) from Pseudomonas fluorescens involved in arylamine oxygenation

Manish Kumar Tiwari, Raushan Kumar Singh, Jung Kul Lee, Huimin Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

We report the mechanistic studies of a FAD:NADH reductase (PrnF) involved in arylamine oxygenation. PrnF catalyzes the reduction of FAD via a sequential ordered bi-bi mechanism with NADH as the first substrate to bind and FADH 2 as the first product to be released. The residues Asp145 and His146 are proposed as catalytic acid/base residues for PrnF based on pH profile and molecular dynamics simulation studies. These studies provide the first detailed account of the mechanism of the flavin reductase involved in arylamine oxygenation.

Original languageEnglish (US)
Pages (from-to)1344-1347
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume22
Issue number3
DOIs
StatePublished - Feb 1 2012

Keywords

  • Arylamine oxygenation
  • FAD:NADH reductase
  • Reaction mechanism

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry
  • Biochemistry

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