Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation

Anqi Wang, Janhavi A. Kolhe, Nate Gioacchini, Imke Baade, William M. Brieher, Craig L. Peterson, Brian C. Freeman

Research output: Contribution to journalArticlepeer-review

Abstract

Movement of chromosome sites within interphase cells is critical for numerous pathways including RNA transcription and genome organization. Yet, a mechanism for reorganizing chromatin in response to these events had not been reported. Here, we delineate a molecular chaperone-dependent pathway for relocating activated gene loci in yeast. Our presented data support a model in which a two-authentication system mobilizes a gene promoter through a dynamic network of polymeric nuclear actin. Transcription factor-dependent nucleation of a myosin motor propels the gene locus through the actin matrix, and fidelity of the actin association was ensured by ARP-containing chromatin remodelers. Motor activity of nuclear myosin was dependent on the Hsp90 chaperone. Hsp90 further contributed by biasing the remodeler-actin interaction toward nucleosomes with the non-canonical histone H2A.Z, thereby focusing the pathway on select sites such as transcriptionally active genes. Together, the system provides a rapid and effective means to broadly yet selectively mobilize chromatin sites. Chromosome reorganization is a central process critical for development and to maintain normal cell homeostasis. Here, Wang et al. report on a pathway able to direct the select movement of chromatin loci in interphase cells using the concerted activities of nuclear molecular chaperones, actin, myosin, and chromatin remodelers.

Original languageEnglish (US)
Pages (from-to)309-320.e5
JournalDevelopmental cell
Volume52
Issue number3
DOIs
StatePublished - Feb 10 2020

Keywords

  • Hsp90
  • chromatin motion
  • chromatin remodeler
  • genome organization
  • molecular chaperone
  • nuclear actin
  • nucleoskeleton

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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