Mechanism and applications of phosphite dehydrogenase

Research output: Contribution to journalReview article

Abstract

Phosphite dehydrogenase catalyzes the NAD+-dependent oxidation of hydrogen phosphonate (common name phosphite) to phosphate in what amounts to a formal phosphoryl transfer reaction from hydride to hydroxide. This review places the enzyme in the context of phosphorus redox metabolism in nature and discusses the results of mechanistic investigations into its reaction mechanism. The potential of the enzyme as a NAD(P)H cofactor regeneration system is discussed as well as efforts to engineer the cofactor specificity of the protein.

Original languageEnglish (US)
Pages (from-to)171-189
Number of pages19
JournalBioorganic Chemistry
Volume33
Issue number3
DOIs
StatePublished - Jun 2005

Fingerprint

NAD
Phosphites
Organophosphonates
Enzymes
Metabolism
Hydrides
Phosphorus
Oxidation-Reduction
Names
Regeneration
Hydrogen
Phosphates
Engineers
Oxidation
Proteins
NAD phosphite oxidoreductase
hydroxide ion

Keywords

  • Cofactor regeneration
  • Dehydrogenase
  • Phosphite
  • Phosphoryl transfer

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Organic Chemistry

Cite this

Mechanism and applications of phosphite dehydrogenase. / Relyea, Heather A.; van der Donk, Wilfred Adrianus.

In: Bioorganic Chemistry, Vol. 33, No. 3, 06.2005, p. 171-189.

Research output: Contribution to journalReview article

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