Abstract

This report describes an approach to extend indefinitely the duration of traction force measurements with cells cultured on soft polyacrylamide gels. Typical observation times in traction force measurements on similar substrates have been limited to 24-48 h, but cell differentiation or responses to external stimuli often occur over much longer periods of several days or weeks. This study describes a method for covalently linking fluorescent marker beads to a polyacrylamide matrix that renders the hydrogels useful for traction force measurements over several days. This approach was validated by comparing the contractility of C2C12 murine skeletal muscle cells prior to myotube formation, after one day in culture, with that of myotubes after 7 days in culture. Measured tractions increased concurrent with the differentiation of C2C12 cells to the contractile, myotube phenotype. Covalent bead linkage thus extends the useful period during which traction force data can be obtained with cells cultured on optically transparent polyacrylamide hydrogels with controlled elastic moduli.

Original languageEnglish (US)
Pages (from-to)40-49
Number of pages10
JournalCellular and Molecular Bioengineering
Volume3
Issue number1
DOIs
StatePublished - Mar 1 2010

Fingerprint

Cell Culture
Force measurement
Traction
Polyacrylates
Cell culture
Cell Culture Techniques
Skeletal Muscle Fibers
Hydrogel
Hydrogels
Cultured Cells
Cell Differentiation
Contractility
Skeletal muscle
Muscle
Cell
Elastic Modulus
Gels
Elastic moduli
Cells
Phenotype

Keywords

  • Cell contractility
  • Differentiation
  • Mechanosensing
  • Polyacrylamide hydrogel
  • Traction force microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Modeling and Simulation

Cite this

Measuring traction forces in long-term cell cultures. / Mann, Cynthia; Leckband, Deborah E.

In: Cellular and Molecular Bioengineering, Vol. 3, No. 1, 01.03.2010, p. 40-49.

Research output: Contribution to journalArticle

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